Subgingival microbiome in smokers and non-smokers in periodontitis: an exploratory study using traditional targeted techniques and a next-generation sequencing
Subgingival microbiome in smokers and non-smokers in periodontitis: an exploratory study using traditional targeted techniques and a next-generation sequencing
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DOI：
10.1111/jcpe.12087
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发表时间：
2013-05-01
影响因子：
6.7
通讯作者：
Zaura, Egija
中科院分区：
文献类型：
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作者：
Bizzarro, Sergio;Loos, Bruno G.;Zaura, Egija
目的比较两种靶向技术与开放式技术在牙周炎患者中的结果，并根据吸烟习惯进行区分。材料与方法30例牙周炎患者（吸烟者15例，非吸烟者15例），采集龈下菌斑标本，进行16 SrRNA基因扩增测序、培养和定量聚合酶链反应（qPCR）检测。结果吸烟者和非吸烟者龈下微生物组的培养和qPCR结果无差异。利用焦磷酸测序，分类为梭杆菌属、普雷沃氏菌属和月形单胞菌属的操作分类单位（OTU）在吸烟者中更丰富，而属于消化球菌属和二氧化碳噬纤维菌属的OTU在非吸烟者中更丰富。主坐标分析确定了两个集群，一个是主要由吸烟者（80%），并揭示了显着较低的分类多样性，较高的附着损失和较高比例的属梭杆菌，沼泽杆菌和脱硫。结论焦磷酸测序结果显示，吸烟者和非吸烟者牙周炎龈下微生物组的组成存在差异。目标技术未识别出这种差异。低分类多样性与较高的疾病严重程度相关，特别是在吸烟者中。这支持了牙周病严重程度中的生态微生物-宿主相互作用的假设。
Aim To compare the results of two targeted techniques to an open-ended technique in periodontitis patients, differentiated on the basis of smoking habit. Materials & Methods Thirty periodontitis patients (15 smokers and 15 non-smokers) provided subgingival plaque samples for 16S rRNA gene amplicon sequencing, culturing and quantitative polymerase chain reaction (qPCR). Results No differences were found in the composition of the subgingival microbiome between smokers and non-smokers with culture and qPCR. With pyrosequencing, operational taxonomic units (OTUs) classified to genera Fusobacterium, Prevotella and Selenomonas were more abundant in smokers, while OTUs belonging to the genera Peptococcus and Capnocytophaga were more abundant in non-smokers. Principal coordinate analysis identified two clusters; one was composed mainly of smokers (80%) and revealed significantly lower taxonomic diversity, higher attachment loss and higher proportion of the genera Fusobacterium, Paludibacter and Desulfobubus. Conclusion In periodontitis, there is a difference in the composition of the subgingival microbiome between smokers and non-smokers, as revealed by pyrosequencing. This difference was not identified by the targeted techniques. Low taxonomic diversity was associated with higher disease severity, especially in smokers. This supports the hypothesis of the ecological microbialhost interaction in the severity of periodontal disease.