Light/dark transition test for mice.
Light/dark transition test for mice.
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DOI：
10.3791/104
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发表时间：
2006-11-13
期刊：
影响因子：
--
通讯作者：
Miyakawa, Tsuyoshi
中科院分区：
文献类型：
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作者：
Takao, Keizo;Miyakawa, Tsuyoshi
虽然所有的小鼠基因组序列已经确定，但我们还不知道这些基因中的大多数的功能。然而，基因靶向技术可用于删除或操纵小鼠中的特定基因。然后，通过对突变小鼠进行行为分析，可以确定给定基因对特定行为的影响。作为突变小鼠行为表型的测试，明/暗转换测试是测量小鼠焦虑样行为的最广泛使用的测试之一。该测试基于小鼠对明亮照明区域的自然厌恶以及它们在新环境中的自发探索行为。该试验对抗焦虑药物治疗敏感。该装置由一个暗室和一个明亮的照明室组成。允许小鼠在两个室之间自由移动。进入明亮房间的次数和在那里停留的时间是老鼠对明亮空间焦虑的指标。为了获得易于复制并与其他突变体进行比较的突变小鼠品系的表型结果，实验室之间的行为试验方法应尽可能相同。然而，实验室之间存在的程序差异使得很难在实验室之间复制或比较结果。在这里，我们提出了我们的协议的光/暗转换测试作为一个电影，使协议的细节可以证明。在我们的实验室里，我们已经使用电影中显示的协议评估了60多个突变小鼠品系。这些数据将作为我们正在建设的公共数据库的一部分公布。方案的可视化将有助于理解整个实验程序的细节，允许跨实验室使用的方案的标准化和使用该测试评估的各种突变小鼠品系的行为表型的比较。
Although all of the mouse genome sequences have been determined, we do not yet know the functions of most of these genes. Gene-targeting techniques, however, can be used to delete or manipulate a specific gene in mice. The influence of a given gene on a specific behavior can then be determined by conducting behavioral analyses of the mutant mice. As a test for behavioral phenotyping of mutant mice, the light/dark transition test is one of the most widely used tests to measure anxiety-like behavior in mice. The test is based on the natural aversion of mice to brightly illuminated areas and on their spontaneous exploratory behavior in novel environments. The test is sensitive to anxiolytic drug treatment. The apparatus consists of a dark chamber and a brightly illuminated chamber. Mice are allowed to move freely between the two chambers. The number of entries into the bright chamber and the duration of time spent there are indices of bright-space anxiety in mice. To obtain phenotyping results of a strain of mutant mice that can be readily reproduced and compared with those of other mutants, the behavioral test methods should be as identical as possible between laboratories. The procedural differences that exist between laboratories, however, make it difficult to replicate or compare the results among laboratories. Here, we present our protocol for the light/dark transition test as a movie so that the details of the protocol can be demonstrated. In our laboratory, we have assessed more than 60 strains of mutant mice using the protocol shown in the movie. Those data will be disclosed as a part of a public database that we are now constructing. Visualization of the protocol will facilitate understanding of the details of the entire experimental procedure, allowing for standardization of the protocols used across laboratories and comparisons of the behavioral phenotypes of various strains of mutant mice assessed using this test.