G8Rotavirus Strains Isolated in the Democratic Republic of Congo Belong to the DS-1-Like Genogroup
G8Rotavirus Strains Isolated in the Democratic Republic of Congo Belong to the DS-1-Like Genogroup
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在刚果民主共和国分离的 G8 轮状病毒株属于 DS-1 样基因组
DOI：
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发表时间：
2006
影响因子：
9.4
通讯作者：
M. Van Ranst
中科院分区：
文献类型：
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作者：
J. Matthijnssens;Mustafizur Rahman;Xue;Thomas Delbeke;I. Arijs;J. Kabue;J. Muyembe;M. Van Ranst
2003年从刚果民主共和国住院患者中分离到几株G8P[6]和G8P[8]轮状病毒。为了研究它们的总体基因组亲缘关系并确定它们属于哪个基因组组，测定了DRC88株(G8P[8])和DRC86株(G8P[6])的全基因组。对这两个非洲G8毒株的基因组比较显示，除VP4外，它们的11个基因片段中有10个几乎相同(核苷酸水平上的同源性为98.9%)，这表明这种罕见的G8P[8]轮状病毒株最近起源于一个普通的G8P[6]毒株和一个具有P[8]特异性的毒株的重组。DRC88和DRC86的11个基因片段中的9个与属于DS-1样(G2P[4])“基因组”的轮状病毒毒株之间存在非常密切的进化关系，并推测了G8P[8]和G8P[6]人类轮状病毒发生之前的几个可能的重新分类事件。由于G2P[4]轮状病毒株的基因对感染人类有很好的适应性，获得一个新的VP7(G8)基因，特别是用P[8](最常见的人类轮状病毒)取代P[6](据信来自动物)，可能使类似DRC88的轮状病毒非常适合成为非洲大陆和世界其他地区的主要人类轮状病毒株和重要病原体。这些发现对轮状病毒疫苗的开发具有重要意义，并强调了仅通过对轮状病毒VP7和VP4基因进行测序来对新轮状病毒株进行分型只为我们提供了有关轮状病毒多样性的冰山一角。
ABSTRACT Several G8P[6] and G8P[8] rotavirus strains were isolated from hospitalized patients in the Democratic Republic of Congo in 2003. To investigate their overall genomic relatedness and to determine to which genogroup they belonged, the complete genomes of strains DRC88 (G8P[8]) and DRC86 (G8P[6]) were determined. Genomic comparison of these two African G8 strains revealed that 10 out of their 11 gene segments, except for VP4, were nearly identical (>98.9% identical at the nucleotide level), suggesting that this rare G8P[8] rotavirus strain originated recently from a reassortment between a common G8P[6] strain and a strain with a P[8] specificity. A very close evolutionary relationship between 9 out of the 11 gene segments of DRC88 and DRC86 and rotavirus strains belonging to the DS-1-like (G2P[4]) “genogroup” was found, and several possible reassortment events preceding the occurrence of G8P[8] and G8P[6] human rotaviruses were hypothesized. Since the genes of G2P[4] rotavirus strains are very well adapted to infect humans, the acquirement of a new VP7 (G8) gene, and especially the replacement of P[6] (believed to be of animal origin) by P[8] (most common in human rotaviruses), might make DRC88-like rotaviruses very well equipped to become a predominant human rotavirus strain and an important pathogen on the African continent and the rest of the world. These findings have important implications for rotavirus vaccine development and highlight that typing of new rotavirus strains by merely sequencing their VP7 and VP4 genes provides us with only the tip of the iceberg regarding rotavirus diversity.