The Combination of Different Carbon Sources Enhances Bacterial Growth Efficiency in Aquatic Ecosystems
The Combination of Different Carbon Sources Enhances Bacterial Growth Efficiency in Aquatic Ecosystems
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DOI：
10.1007/s00248-013-0277-1
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发表时间：
2013-11-01
影响因子：
3.6
通讯作者：
Farjalla, Vinicius F.
中科院分区：
文献类型：
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作者：
Fonte, Ellen S.;Amado, Andre M.;Farjalla, Vinicius F.
溶解有机碳（DOC）库是由不同碳源的有机碳化合物组成的。这些来源中的每一种都可能支持不同的细菌生长速率，但很少有研究专门分析不同碳源组合对细菌代谢的影响。在这项研究中，我们评估了几个代谢参数的响应，包括细菌生物量生产（BP），细菌呼吸（BR），细菌生长效率（BGE），和细菌群落结构，对存在的三个DOC源单独和组合。我们假设，不同DOC源的混合物将增加细菌的碳利用效率（BGE）。我们建立了一个全因子替代设计（7个处理），其中DOC来源的数量和身份对细菌代谢的影响进行了评估。我们计算了基于单一DOC处理的组合DOC处理的预期代谢率，并观察到对BP的正相互作用，对BR的负相互作用，以及因此对BGE的正相互作用。细菌群落组成似乎有一个小的影响，在细菌代谢的差异治疗。我们的数据表明，DOC源的混合物导致更有效的生物利用碳。本研究为不同DOC源的混合是影响细菌在水生态系统碳通量中作用的关键因素提供了强有力的证据。
The dissolved organic carbon (DOC) pool is composed of several organic carbon compounds from different carbon sources. Each of these sources may support different bacterial growth rates, but few studies have specifically analyzed the effects of the combination of different carbon sources on bacterial metabolism. In this study, we evaluated the response of several metabolic parameters, including bacterial biomass production (BP), bacterial respiration (BR), bacterial growth efficiency (BGE), and bacterial community structure, on the presence of three DOC sources alone and in combination. We hypothesized that the mixture of different DOC sources would increase the efficiency of carbon use by bacteria (BGE). We established a full-factorial substitutive design (seven treatments) in which the effects of the number and identity of DOC sources on bacterial metabolism were evaluated. We calculated the expected metabolic rates of the combined DOC treatments based on the single-DOC treatments and observed a positive interaction on BP, a negative interaction on BR, and, consequently, a positive interaction on BGE for the combinations. The bacterial community composition appeared to have a minor impact on differences in bacterial metabolism among the treatments. Our data indicate that mixtures of DOC sources result in a more efficient biological use of carbon. This study provides strong evidence that the mixture of different DOC sources is a key factor affecting the role of bacteria in the carbon flux of aquatic ecosystems.