Investigation of transfusion transmission of a WA1-type babesial parasite to a premature infant in California
Investigation of transfusion transmission of a WA1-type babesial parasite to a premature infant in California
复制标题
DOI：
10.1046/j.1537-2995.2002.00245.x
复制
发表时间：
2002-11-01
期刊：
影响因子：
2.9
通讯作者：
Conrad, PA
中科院分区：
文献类型：
--
作者：
Kjemtrup, AM;Lee, B;Conrad, PA
背景：加利福尼亚州的一名早产儿因感染原虫巴贝斯虫而出现呼吸窘迫。婴儿接受了两次输血，一次来自父亲，另一次来自匿名捐赠者(捐赠者A)。这项研究描述了确定感染婴儿的巴贝斯虫的来源和种类所需的随访。研究设计和方法：在婴儿患病时，评估婴儿、父亲和母亲的全血是否感染巴贝斯虫。在疑似献血给婴儿2个月后，对捐赠者A的全血进行了类似的评估。样本通过血液涂片检查、血清学、聚合酶链式反应和仓鼠接种进行检测。对回收的巴贝斯虫进行DNA扩增、18S基因测序和系统发育分析。结果：从婴儿体内分离到WA1型巴贝斯虫。父母都不是感染源。血清学和仓鼠接种证实献血员感染WA1型巴贝斯虫。婴儿和献血员分离株的18S基因DNA序列100%相同。结论：WA1型巴贝斯虫感染在献血员中可能很难检测到，因为这种感染可能是亚临床的。这是第二例WA1型巴贝斯虫通过输血传播，也是第一例婴儿传播。美国西部的医生应该考虑巴贝斯症是输血后引起非特异性发热性疾病的可能原因。
Background: A premature infant in California developed respiratory distress associated with infection with a protozoal parasite, Babesia. The infant had received two blood transfusions, one from the father and one from an anonymous donor (Donor A). This study describes the follow-up required to identify the source and species of Babesia that infected the infant.Study design and methods: At the time of the infant's illness, whole blood from the infant, father, and mother was evaluated for Babesia infection. Similar evaluation of whole blood from Donor A was performed 2 months after the suspected donation to the infant. Samples were tested using blood smear examination, serology, PCR, and hamster inoculation. Identity of the recovered Babesia parasites was confirmed by DNA amplification by PCR, genetic sequencing of the 18S gene, and phylogenetic analysis.Results: WA1-type Babesia was recovered from the infant. Neither parent was the source of infection. Serology and hamster inoculation confirmed WA1-type Babesia infection in Donor A. DNA sequences of the 18S gene from the infant and donor isolates were 100% identical.Conclusion: WA1-type Babesia infections may be difficult to detect among blood donors because such infections can be subclinical. This is the second WA1-type Babesia transmission via blood transfusion and the first in an infant. Physicians in the western United States should consider Babesia as a possible cause of nonspecific febrile illness after a blood transfusion.