Composition and Diversity of Bacterial Community on the Ocular Surface of Patients With Meibomian Gland Dysfunction
Composition and Diversity of Bacterial Community on the Ocular Surface of Patients With Meibomian Gland Dysfunction
复制标题
睑板腺功能障碍患者眼表细菌群落的组成和多样性
DOI：
10.1167/iovs.19-27719
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发表时间：
2019-11-01
影响因子：
4.4
通讯作者：
Huang, Yusen
中科院分区：
文献类型：
--
作者：
Dong, Xiaojin;Wang, Yuqian;Huang, Yusen
目的。方法：采用165rDNA测序方法，对47例眉板腺功能障碍(MGD)患者和42例年龄、性别相匹配的非MGD患者(对照组)进行眼表细菌群落组成和多样性的研究。样本从每个参与者随机选择的一只眼睛的上下结膜囊中采集。通过对样本中165个rDNA基因的高变区进行测序，比较不同组间在分类和多样性上的差异。结果主坐标分析显示重度MGD组与其他组结膜囊细菌群落具有明显的聚集性。在门水平上，MGD组细菌相对丰度(31.70%vs.19.67%)和变形杆菌(27.46%vs.14.66%)显著高于对照组(P<；0.05，Mann-Whitney U)，放线菌丰度(34.17%vs.56.98%)低于对照组(P<；0.05，Mann-Whitney U)。在属水平上，MGD患者葡萄球菌(20.71%vs.7.88%)和Spbingon：ONAS(5.73%vs.0.79%)显著高于对照组(P<；0.05，Mann-Whitney U)，而棒状杆菌(20.22%vs.46.43%)显著低于对照组(P<；0.05，Mann-Whitney U)。葡萄球菌丰度与结膜囊细菌数呈正相关(r=0.650，P<；0.001)。结论：MGD患者结膜囊内存在不同程度的细菌菌群失调。葡萄球菌、棒状杆菌和鞘氨醇单胞菌可能在MGD的病理生理学中起作用。
PURPOSE. To investigate the composition and diversity of bacterial community on the ocular surface of patients with meibomian gland dysfunction (MGD) via 165 rDNA sequencing.METHODS. Forty-seven patients with MGD, who were divided into groups of mild, moderate, and severe MGD, and 42 sex- and age-matched participants without MGD (control group) were enrolled. Samples were collected from the upper and lower conjunctival sac of one randomly chosen eye of each participant. Through sequencing the hypervariable region of 165 rDNA gene obtained from samples, differences in the taxonomy and diversity between groups were compared.RESULTS. Principle coordinate analysis showed significantly distinct clustering of the conjunctival sac bacterial community between the severe MGD group and the other groups. At the phylum level, the relative abundances of Firmicutes (31.70% vs. 19.67%) and Proteobacteria (27.46% vs. 14.66%) were significantly higher (P < 0.05, Mann-Whitney U), and the abundance of Actinobacteria (34.17% vs. 56.98%) was lower in MGD than controls (P < 0.05, Mann-Whitney U). At the genus level, the abundances of Staphylococcus (20.71% vs. 7.88%) and Spbingon:onas (5.73% vs. 0.79%) in patients with MGD were significantly higher than the controls (P < 0.05, Mann-Whitney U), while the abundance of Corynebacterium (20.22% vs. 46.43%) was significantly lower (P < 0.05, Mann-Whitney U). The abundance of Staphylococcus was positively correlated with the meiboscores in patients with MGD (r = 0.650, P < 0.001, Spearman).CONCLUSIONS. Patients with MGD can have various degrees of bacterial microbiota imbalance in the conjunctival sac. Staphylococcus, Corynebacteriurn, and Sphingomonas may play roles in the pathophysiology of MGD.