Cysteamine improves growth performance and gastric ghrelin expression in preweaning piglets
Cysteamine improves growth performance and gastric ghrelin expression in preweaning piglets
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DOI：
10.1016/j.domaniend.2011.12.003
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发表时间：
2012-05-01
影响因子：
2.1
通讯作者：
Zhao, R.
中科院分区：
文献类型：
--
作者：
Du, G.;Shi, Z.;Zhao, R.
本研究旨在研究半胱胺对断奶前仔猪生长性能及胃内ghrelin mRNA表达的影响。将12窝新生仔猪随机分配至对照组和给药组。从15日龄开始，对照组饲喂基础蠕动日粮，试验组在基础日粮中添加120 mg/kg半胱胺，直至35日龄断奶。记录体重增加、蠕动饲料消耗和腹泻率，并收集胃粘膜组织用于定量mRNA表达。为了评估半胱胺对胃饥饿素表达的直接影响，将从35日龄仔猪分离的胃粘膜细胞的原代培养物分别暴露于0、1、10和100 μ g/mL的半胱胺20小时。日粮中添加半胱胺可显著提高断奶前仔猪的平均日采食量和增重（P < 0.05），并降低腹泻率。35日龄时，半胱胺处理组仔猪胃粘膜ghrelin和gastrin mRNA表达显著增加（P < 0.05），生长抑素mRNA表达显著降低（P < 0.05）。半胱胺组血清胃泌素水平显著高于对照组（P < 0.05）。在体外，半胱胺在10 μ g/mL的浓度下显著增加胃粘膜细胞中ghrelin mRNA的表达。因此，日粮中添加半胱胺能够有效改善断奶前仔猪的生长性能和健康状况，其机制可能与半胱胺在体内和体外均能促进胃Ghrelin mRNA的表达有关。(C)2012 Elsevier Inc. All rights reserved.
The aim of the present study was to investigate the effect of cysteamine on growth performance of preweaning piglets and gastric expression of ghrelin mRNA in vivo and in vitro. Twelve litters of newborn piglets were allocated randomly to control and treatment groups. From 15 d of age, piglets in the control group were fed basal creep diet, whereas the treatment group received basal diet supplemented with 120 mg cysteamine per kg of diet until weaning on 35 d of age. Body weight gain, creep feed consumption, and diarrhea rates were recorded, and gastric mucosal tissues were collected for quantifying mRNA expression. To evaluate the direct effect of cysteamine on gastric ghrelin expression, primary cultures of gastric mucosal cells isolated from 35-d-old piglets were exposed to cysteamine for 20 h at 0, 1, 10, and 100 mu g/mL, respectively. Dietary cysteamine increased (P < 0.05) average daily creep feed consumption and BW gain in preweaning pigs, which was accompanied by reduction in diarrhea rates. At 35 d of age, piglets treated with cysteamine showed increased (P < 0.05) ghrelin and gastrin and decreased (P < 0.05) somatostatin mRNA expression in gastric mucosa. Moreover, dietary cysteamine treatment increased serum concentration of gastrin (P < 0.05). In vitro, cysteamine significantly increased ghrelin mRNA expression in gastric mucosal cells at the concentration of 10 mu g/mL. In conclusion, dietary cysteamine is effective in improving the growth performance and health condition of preweaning piglets, which is associated with its stimulatory effects on gastric ghrelin mRNA expression both in vivo and in vitro. (C) 2012 Elsevier Inc. All rights reserved.