Gene expression underlying adaptive variation in Heliconius wing patterns:: non-modular regulation of overlapping cinnabar and vermilion prepatterns
Gene expression underlying adaptive variation in Heliconius wing patterns:: non-modular regulation of overlapping cinnabar and vermilion prepatterns
复制标题
DOI：
10.1098/rspb.2007.1115
复制
发表时间：
2008-01-07
影响因子：
4.7
通讯作者：
Nagy, Lisa M.
中科院分区：
文献类型：
--
作者：
Reed, Robert D.;McMillan, W. Owen;Nagy, Lisa M.
蝴蝶螺旋藻Erato模拟翅膀模式的地理变异是适应性多态的教科书例子；然而，关于这种变异是如何在发育中控制的，人们知之甚少。利用基因芯片和定量聚合酶链式反应，我们鉴定和比较了可能与红/黄前翅带多态相关的候选基因的表达。我们发现，编码色素合成酶朱砂和朱砂的转录本分别显示了模式相关和多态相关的表达模式。朱砂的表达与前翼带相关，与色素颜色无关，提供了第一个已知与主要赫利柯斯颜色模式相关的基因表达模式。相反，在红带蝴蝶中，朱红的表达随着时间的推移而在空间上发生变化，但在黄带蝴蝶中没有表达，这表明该基因的调控可能与红色/黄色多态有关。此外，我们还发现，黄色素3-羟基犬尿氨酸是从血淋巴中结合到翼鳞中的，而不是原位合成的。我们认为Helconius颜色模式的某些方面是由色素基因转录前模式的时空重叠决定的，并推测朱红调节的进化变化可能是适应性颜色模式多态的部分基础。
Geographical variation in the mimetic wing patterns of the butterfly Heliconius erato is a textbook example of adaptive polymorphism; however, little is known about how this variation is controlled developmentally. Using microarrays and qPCR, we identified and compared expression of candidate genes potentially involved with a red/yellow forewing band polymorphism in H. erato. We found that transcripts encoding the pigment synthesis enzymes cinnabar and vermilion showed pattern- and polymorphism-related expression patterns, respectively. cinnabar expression was associated with the forewing band regardless of pigment colour, providing the first gene expression pattern known to be correlated with a major Heliconius colour pattern. In contrast, vermilion expression changed spatially over time in red-banded butterflies, but was not expressed at detectable levels in yellow-banded butterflies, suggesting that regulation of this gene may be involved with the red/yellow polymorphism. Furthermore, we found that the yellow pigment, 3-hydroxykynurenine, is incorporated into wing scales from the haemolymph rather than being synthesized in situ. We propose that some aspects of Heliconius colour patterns are determined by spatio-temporal overlap of pigment gene transcription prepatterns and speculate that evolutionary changes in vermilion regulation may in part underlie an adaptive colour pattern polymorphism.