Cdx2 represses Oct4 function via inducing its proteasome-dependent degradation in early porcine embryos
Cdx2 represses Oct4 function via inducing its proteasome-dependent degradation in early porcine embryos
复制标题
Cdx2 通过诱导早期猪胚胎中蛋白酶体依赖性降解来抑制 Oct4 功能
DOI：
10.1016/j.ydbio.2015.12.014
复制
发表时间：
2016-02-01
影响因子：
2.7
通讯作者：
Liu, Zhonghua
中科院分区：
文献类型：
--
作者：
Bou, Gerelchimeg;Liu, Shichao;Liu, Zhonghua
内细胞团特异性因子OCT 4和滋养外胚层特异性因子CDX 2的相互抑制促进小鼠第一谱系分离。在小鼠胚胎干细胞（ES细胞）中的研究表明，它们可以相互结合到彼此的调控区以抑制转录，此外它们还形成蛋白质复合物以相互拮抗。然而，到目前为止，Oct4和Cdx2在其他哺乳动物早期胚胎中的分子相互作用尚未研究。Cdx 2在早期猪胚胎中的过表达表明，Cdx 2既不通过转录抑制也不通过形成抑制复合物来抑制OCT 4，而是促进OCT 4核输出和蛋白酶体降解。本研究结果为阐明CDX2与Oct4在小鼠以外哺乳动物胚胎中的相互作用机制提供了重要线索。(C)2015 Elsevier Inc. All rights reserved.
Reciprocal repression of inner cell mass specific factor OCT4 and trophectoderm specific factor CDX2 promotes mouse first lineage segregation. Studies in mouse embryonic stem (ES) cells revealed that they bind to each other's regulatory regions to reciprocally suppress transcription, additionally they form protein complex for mutual antagonism. However, so far the molecular interaction of Oct4 and Cdx2 in other mammal's early embryo is not yet investigated. Here, over-expression of Cdx2 in early porcine embryo showed CDX2 represses Oct4 through neither the transcriptional repression nor forming repressive complex, but promoting OCT4 nuclear export and proteasomal degradation. The results showed novel molecular regulation of CDX2 on Oct4, and provided important clues for clarifying the mechanism of interaction between CDX2 and Oct4 in embryo of mammals other than mouse. (C) 2015 Elsevier Inc. All rights reserved.