On the mechanism of anaphase spindle elongation in Diatoma vulgare.
On the mechanism of anaphase spindle elongation in Diatoma vulgare.
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DOI：
10.1083/jcb.74.2.377
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发表时间：
1977-08
期刊：
影响因子：
--
通讯作者：
Tippit DH
中科院分区：
文献类型：
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作者：
McDonald K;Pickett-Heaps JD;McIntosh JR;Tippit DH
从连续切片中重建了五个分裂细胞（一个中期，三个后期，和一个末期）的Diatoma vulgare的中心纺锤体。每个纺锤体由两个几乎完全由极微管组成的半纺锤体组成。除末期外，各期均有少量的连续微管和游离微管。半纺锤体在中央纺锤体的中部相互交错，形成一个重叠区，其中一极的微管与另一极的微管混合。在分裂中期，重叠区相当广泛，但随着伸长的进行，纺锤体两极分开，重叠的长度减少，因为很少有微管足够长，可以从两极到达交错区。在末期，只有少数小管足够长以在中部重叠。重叠区长度减少的同时，同一区域的微管间基质染色密度增加。这些形态学的变化可以很容易地解释，假设区的微管之间的机械化学相互作用的重叠区，这导致在一个滑动分开的两个半纺锤体。
Central spindles from five dividing cells (one metaphase, three anaphase, and one telophase) of Diatoma vulgare were reconstructed from serial sections. Each spindle is made up of two half-spindles that are composed almost entirely of polar microtubules. A small percentage of continuous microtubules and free microtubules were present in every stage except telophase. The half-spindles interdigitate at the midregion of the central spindle, forming a zone of overlap where the microtubules from one pole intermingle with those of the other. At metaphase the overlap zone is fairly extensive, but as elongation proceeds, the spindle poles move apart and the length of the overlap decreases because fewer microtubules are sufficiently long to reach from the pole to the zone of interdigitation. At telophase, only a few tubules are long enough to overlap at the midregion. Concurrent with the decrease in the length of the overlap zone is an increase in the staining density of the intermicrotubule matrix at the same region. These changes in morphology can most easily be explained by assuming zone mechanochemical interaction between microtubules in the overlap zone which results in a sliding apart of the two half-spindles.