A side order of stem cells: The SP phenotype
A side order of stem cells: The SP phenotype
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DOI：
10.1634/stemcells.2005-0116
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发表时间：
2006-01-01
期刊：
影响因子：
5.2
通讯作者：
Little, MH
中科院分区：
文献类型：
--
作者：
Challen, GA;Little, MH
小鼠造血干细胞（HSC）的一个定义性质是用Hoechst 33342和罗丹明123染色后的低荧光。当单独使用或与针对干细胞表位的抗体组合使用时，这些染料已被证明是HSC纯化和表征中非常强大的工具。根据Hoechst红与Hoechst蓝双变量荧光激活细胞分选点图中的典型特征，Hoechst低细胞被描述为侧群（SP）细胞。最近，令人兴奋的发现，推定的干细胞从实体组织也可能具有这种SP表型。基于这种相同的染料流出现象，SP细胞现已从多种哺乳动物组织中分离出来，并且在许多情况下，这种细胞群已显示含有明显的多能干细胞。尚待明确解决的是细胞融合是否占这种感知SP多能性。事实上，如果Hoechst染色后的低荧光是造血干细胞和器官特异性干细胞共有的表型，那么所有驻留的组织SP细胞是否都具有骨髓起源，或者SP表型是否是所有干细胞共有的特性？根据进一步的分析，SP表型可以证明是非常宝贵的居民组织干细胞的初始分离的情况下，确定的细胞表面标志物，并可能有广泛的应用在干细胞生物学，从纯化新的干细胞群体的自体干细胞疗法的发展。
A defining property of murine hematopoietic stein cells (HSCs) is low fluorescence after staining with Hoechst 33342 and Rhodamine 123. These dyes have proven to be remarkably powerful tools in the purification and characterization of HSCs when used alone or in combination with antibodies directed against stem cell epitopes. Hoechst low cells are described as side population (SP) cells by virtue of their typical profiles in Hoechst red versus Hoechst blue bivariate fluorescent-activated cell sorting dot plots. Recently, excitement has been generated by the findings that putative stem cells from solid tissues may also possess this SP phenotype. SP cells have now been isolated from a wide variety of mammalian tissues based on this same dye efflux phenomenon, and in many cases this cell population has been shown to contain apparently multipotent stem cells. What is yet to be clearly addressed is whether cell fusion accounts for this perceived SP multipotency. Indeed, if low fluorescence after Hoechst staining is a phenotype shared by hematopoietic and organ-specific stem cells, do all resident tissue SP cells have bone marrow origins or might the SP phenotype be a property common to all stem cells? Subject to further analysis, the SP phenotype may prove invaluable for the initial isolation of resident tissue stem cells in the absence of definitive cell-surface markers and may have broad-ranging applications in stem cell biology, from the purification of novel stem cell populations to the development of autologous stem cell therapies.