Kinetics of plasma membrane electron transport in a pulmonary endothelial cell-column.
Kinetics of plasma membrane electron transport in a pulmonary endothelial cell-column.
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肺内皮细胞柱中质膜电子传递的动力学。
DOI：
10.1114/1.83
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发表时间：
1998
影响因子：
3.8
通讯作者：
Dawson,CA
中科院分区：
文献类型：
--
作者：
Olson,LE;Merker,MP;Bongard,RD;Brantmeier,BM;Audi,SH;Linehan,JH;Dawson,CA
噻嗪染料如甲苯胺蓝O（TBO）在管腔内皮表面被还原。本研究的目的是确定培养的内皮细胞中这种反应的速率。使用多指示剂稀释法测量含TBO的推注通过色谱柱（色谱柱填充有生长在微载体珠上的牛肺动脉内皮细胞）时的反应动力学。将含有TBO和惰性细胞外参考指示剂（FITC-葡聚糖）的团注入细胞柱的上游，并使用在线光电检测在下游测量指示剂浓度。研究了柱流速、PO 2和TBO浓度对分离效果的影响。在通过细胞柱时减少的TBO的分数随着流量的增加而减少，表明反应速率而不是TBO递送控制TBO减少。在研究范围内，TBO减少的分数不随PO 2或剂量而变化。TBO减少是这些细胞的稳态TBO螯合的约10倍，这沿着缺乏PO 2效应表明快速减少速率不是稳态螯合中的速率限制步骤。
Thiazine dyes such as toluidine blue O (TBO) are reduced at the luminal endothelial surface. The purpose of this study was to determine the rate of this reaction in endothelial cells in culture. A multiple indicator dilution method was used to measure the reaction kinetics during transient passage of a TBO-containing bolus through a chromatographic column filled with bovine pulmonary arterial endothelial cells grown on microcarrier beads (cell-column). A bolus containing TBO and an inert extracellular reference indicator (FITC-Dextran) was injected upstream of the cell-column, and the indicator concentrations were measured downstream using on-line photodetection. The effects of column flow rate, PO2, and TBO concentration were studied. The fraction of TBO reduced upon passage through the cell-column decreased with increasing flow indicating that the reaction rate rather than TBO delivery controlled TBO reduction. The fraction of TBO reduced did not change with PO2or dose in the ranges studied. TBO reduction was about 10 times that for steady state TBO sequestration by these cells which, along with the lack of a PO2effect indicates that the rapid rate of reduction is not the rate-limiting step in steady state sequestration.PAC98: 8722Fy, 8220-w