Effects of heat shock on survival, proliferation and differentiation of mouse neural stem cells
Effects of heat shock on survival, proliferation and differentiation of mouse neural stem cells
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DOI：
10.1016/j.neures.2013.11.005
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发表时间：
2014-02-01
影响因子：
2.9
通讯作者：
Inoue, Nobuo
中科院分区：
文献类型：
--
作者：
Omori, Hiroyuki;Otsu, Masahiro;Inoue, Nobuo
怀孕期间的高热是生殖问题的一个重要原因，这些问题包括流产和中枢神经系统（CNS）先天性缺陷，包括神经管缺陷和小头畸形。神经干细胞（NSC）可以增殖并分化为神经元和神经胶质细胞，在中枢神经系统的形成中发挥关键作用。在这里，我们研究了热休克对小鼠胚胎干细胞来源的同质增殖 NSC 的影响。 42℃热激20分钟后，增殖中的NSC继续增殖，但基因表达、细胞存活和增殖方面观察到细微变化。相比之下，43摄氏度的热休克会引起多种反应：编码热休克蛋白（HSP）的基因上调、诱导细胞凋亡、暂时抑制细胞增殖和延迟分化。最后，44摄氏度的热休克效应非常严重，几乎所有细胞都消失了，剩下的细胞失去了增殖和分化的能力。热休克对神经干细胞的这些温度依赖性影响可能对于阐明怀孕期间高温导致各种生殖问题的机制很有价值。 (C) 2013 Elsevier Ireland Ltd 和日本神经科学学会。版权所有。
Hyperthermia during pregnancy is a significant cause of reproductive problems ranging from abortion to congenital defects of the central nervous system (CNS), including neural tube defects and microcephaly. Neural stem cells (NSCs) can proliferate and differentiate into neurons and glia, playing a key role in the formation of the CNS. Here, we examined the effects of heat shock on homogeneous proliferating NSCs derived from mouse embryonic stem cells. After heat shock at 42 degrees C for 20 min, the proliferating NSCs continued to proliferate, although subtle changes were observed in gene expression and cell survival and proliferation. In contrast, heat shock at 43 degrees C caused a variety of responses: the up-regulation of genes encoding heat shock proteins (HSP), induction of apoptosis, temporal inhibition of cell proliferation and retardation of differentiation. Finally, effects of heat shock at 44 degrees C were severe, with almost all cells disappearing and the remaining cells losing the capacity to proliferate and differentiate. These temperature-dependent effects of heat shock on NSCs may be valuable in elucidating the mechanisms by which hyperthermia during pregnancy causes various reproductive problems. (C) 2013 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.