Identification of calnexin as a binding protein for Amadori-modified glycated albumin.
Identification of calnexin as a binding protein for Amadori-modified glycated albumin.
复制标题
鉴定钙连接蛋白作为阿马多里修饰的糖化白蛋白的结合蛋白。
DOI：
10.1006/bbrc.2001.4982
复制
发表时间：
2001
影响因子：
3.1
通讯作者：
Cohen,MP
中科院分区：
文献类型：
--
作者：
Wu,VY;Shearman,CW;Cohen,MP
Albumin modified by Amadori glucose adducts (glycated albumin) selectively binds to glomerular mesangial cells and triggers signal transduction processes that modulate cellular function. To identify glycated albumin binding proteins, we applied membrane extracts prepared from murine mesangial cells to a column of lysine-Sepharose followed by application to an affinity column of fructosyllysine-Sepharose. This procedure yielded an approximately 90 kDa polypeptide that immunoreacted with Amadori-modified but not carbohydrate-free albumin. MALDI mass fingerprinting matched 9 out of 25 peptides with calnexin, and amino acid analysis showed homology with this transmembrane calcium-binding protein of the calreticulin family. These results indicate that one of the mesangial cell receptors for glycated albumin is a calnexin-like protein.
