Eight high-quality genomes reveal pan-genome architecture and ecotype differentiation of Brassica napus
Eight high-quality genomes reveal pan-genome architecture and ecotype differentiation of Brassica napus
复制标题
八个高质量基因组揭示了甘蓝型油菜的泛基因组结构和生态型分化
DOI：
10.1038/s41477-019-0577-7
复制
发表时间：
2020-01-13
期刊：
影响因子：
18
通讯作者：
Guo, Liang
中科院分区：
文献类型：
--
作者：
Song, Jia-Ming;Guan, Zhilin;Guo, Liang
油菜（Brassica napus）是世界上第二重要的油籽作物，但其巨大表型变异背后的遗传多样性仍未得到充分研究。在这里，我们报道了8个b的测序、从头组装和注释。napusaccessions。通过泛基因组比较分析，鉴定出数百万个小变异和77.2-149.6个大碱基存在和缺失变异（pav）。超过9.4%的基因包含大效应突变或结构变异。基于pav的全基因组关联研究（PAV-GWAS）在以ZS11（参考系）为供体的巢式关联定位群体中直接发现了单核苷酸多态性的GWAS （SNP-GWAS）未检测到的单株长度、种子重量和开花时间的因果结构变异，表明PAV-GWAS在鉴定性状关联方面与SNP-GWAS是互补的。进一步分析表明，3个降花基因的pav值与开花时间和生态型分化密切相关。本研究为更好地了解b基因的基因组结构和加速b基因的遗传改良提供了资源。显著。
Rapeseed (Brassica napus) is the second most important oilseed crop in the world but the genetic diversity underlying its massive phenotypic variations remains largely unexplored. Here, we report the sequencing, de novo assembly and annotation of eightB. napusaccessions. Using pan-genome comparative analysis, millions of small variations and 77.2–149.6 megabase presence and absence variations (PAVs) were identified. More than 9.4% of the genes contained large-effect mutations or structural variations. PAV-based genome-wide association study (PAV-GWAS) directly identified causal structural variations for silique length, seed weight and flowering time in a nested association mapping population with ZS11 (reference line) as the donor, which were not detected by single-nucleotide polymorphisms-based GWAS (SNP-GWAS), demonstrating that PAV-GWAS was complementary to SNP-GWAS in identifying associations to traits. Further analysis showed that PAVs in threeFLOWERING LOCUS Cgenes were closely related to flowering time and ecotype differentiation. This study provides resources to support a better understanding of the genome architecture and acceleration of the genetic improvement ofB. napus.