Carriage of Clostridioides difficile in healthy infants in the community of Handan, China: A 1-year follow-up study
Carriage of Clostridioides difficile in healthy infants in the community of Handan, China: A 1-year follow-up study
复制标题
DOI：
10.1016/j.anaerobe.2020.102295
复制
发表时间：
2021-02-01
期刊：
影响因子：
2.3
通讯作者：
Zhao, Jian-Hong
中科院分区：
文献类型：
--
作者：
Cui, Qing-Qing;Yang, Jing;Zhao, Jian-Hong
目的：艰难梭菌可能在无症状的情况下长期定植于健康婴儿和幼儿中。艰难梭菌基因型以及定植率和决定因素存在很大差异，并且在不同国家和地区之间存在差异。进行为期一年的随访研究，以确定艰难梭菌肠道定植的发生率、动力学和影响因素。方法：对邯郸市与父母同住的 29 名健康婴儿（14 名女孩和 15 名男孩）从出生到 1 岁，特别是从 2014 年 10 月至 2015 年 12 月进行跟踪调查。通过 PCR 核糖分型对艰难梭菌分离株进行分型，并分析是否存在毒素。结果：在生命第一年的随访研究期间，29 名入组婴儿中有 20 名感染了艰难梭菌。共获取粪便样本437份，其中111份（25.4%）样本含有艰难梭菌，其中产毒菌株79份（71.2%）。产毒菌株包含 6 种 PCR 核糖核酸型，其中 2 种 PCR 核糖核酸型被鉴定为非产毒菌株。结论：我们的研究表明，在 12 个月期间，艰难梭菌定植随着年龄的增长而增加，婴儿中艰难梭菌分离株的主要产毒类型是长期定植的菌株。喂养模式可能会影响艰难梭菌定植的动态进展。 (C) 2020 作者。由爱思唯尔有限公司出版
Objective: Clostridioides difficile may colonize healthy infants and young children asymptomatically and for the long-term. C. difficile genotypes and the rate and determinants of colonization differ substantially and vary among countries and regions. A 1-year follow-up study was performed to determine the incidence, kinetics and influencing factors of C. difficile intestinal colonization.Methods: Twenty-nine healthy infants (14 girls and 15 boys) living at home with their parents in Handan City were followed by survey from birth to 1 year of age, specifically from October 2014 through December 2015. C. difficile isolates were typed by PCR ribotyping and analyzed for the presence of toxin genes.Results: During the follow-up study period in the first year of life, 20 of the 29 total enrolled infants acquired C. difficile. A total of 437 fecal samples were obtained, and 111 (25.4%) samples contained C. difficile, including 79 (71.2%) toxigenic strains. The toxigenic isolates comprised six PCR ribotypes, and two PCR ribotypes were identified as nontoxigenic strains.Conclusion: Our study showed that C. difficile colonization increase with age during the 12-month period, and the dominant toxigenic types of C. difficile isolates in infants were those involved in long-term colonization. Feeding patterns may affect the dynamic progress of C. difficile colonization. (C) 2020 The Author(s). Published by Elsevier Ltd.