Yeast mitochondrial ADP/ATP carriers are monomeric in detergents
Yeast mitochondrial ADP/ATP carriers are monomeric in detergents
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DOI：
10.1073/pnas.0607640103
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发表时间：
2006-10-31
影响因子：
11.1
通讯作者：
Kunji, Edmund R. S.
中科院分区：
文献类型：
--
作者：
Bamber, Lisa;Harding, Marilyn;Kunji, Edmund R. S.
线粒体载体被广泛认为是细胞器内壁和洗涤剂中的同型二聚体。测定了不同洗涤剂中酵母ADP/ATP载体的洗涤剂和蛋白质-洗涤剂胶束的尺寸和分子质量。测定了载体在膜中点的半径、平均半径、斯托克斯半径、分子质量和排除体积。这些参数与已知的牛ADP/ATP载体的结构模型一致，证明酵母线粒体ADP/ATP载体在洗涤剂中是单体的。因此，必须考虑底物输运模型，其中载体作为单体而不是作为二聚体运作。
Mitochondrial carriers are believed widely to be homodimers both in the inner membrane of the organelle and in detergents. The dimensions and molecular masses of the detergent and protein-detergent micelles were measured for yeast ADP/ATP carriers in a range of different detergents. The radius of the carrier at the midpoint of the membrane, its average radius, its Stokes' radius, its molecular mass, and its excluded volume were determined. These parameters are consistent with the known structural model of the bovine ADP/ATP carrier and they demonstrate that the yeast mitochondrial ADP/ATP carriers are monomeric in detergents. Therefore, models of substrate transport have to be considered in which the carrier operates as a monomer rather than as a dimer.