Template-assisted fabrication of protein nanocapsules
Template-assisted fabrication of protein nanocapsules
复制标题
蛋白质纳米胶囊的模板辅助制造
DOI：
10.1007/s11051-008-9387-y
复制
发表时间：
2009
影响因子：
2.5
通讯作者：
T. Kowalik
中科院分区：
文献类型：
--
作者：
S. Dougherty;Jianyu Liang;T. Kowalik
生物纳米材料最近开始引起人们的极大兴趣，并有可能彻底改变生物医学研究。纳米颗粒、纳米胶囊和纳米管结构正在成为药物和基因传递的有吸引力的选择。运载工具的尺寸极大地影响了细胞的摄取，因此精确控制纳米载体的直径和长度以低成本制造均匀的纳米颗粒是非常必要的。碳纳米管在药物和基因传递领域显示出巨大的潜力。然而，它们的不溶性和细胞毒性可能会严重推迟FDA的批准。一个理想的替代方案是用生物材料制造纳米结构，如蛋白质、多肽或脂质体，这些材料已经得到FDA的批准。在这篇文章中，我们展示了蛋白质纳米胶囊的制备与两端密封使用模板辅助交替浸泡法结合控制切割。制备了直径、壁厚和长度可控的葡萄糖氧化酶纳米胶囊，并用扫描电镜和透射电镜对其进行了表征。用紫外光谱法测定了葡萄糖氧化酶纳米胶囊处理后的生化活性。我们未来的工作将探索适合药物包封和细胞摄取的蛋白质，并将重点放在优化切割过程上，以精确控制纳米胶囊的长度。
Bionanomaterials have recently begun to spark a great amount of interest and could potentially revolutionize biomedical research. Nanoparticles, nanocapsules, and nanotubular structures are becoming attractive options in drug and gene delivery. The size of the delivery vehicles greatly impacts cellular uptake and makes it highly desirable to precisely control the diameter and length of nanocarriers to make uniform nanoparticles at low cost. Carbon nanotubes have shown great potential within the field of drug and gene delivery. However, their insolubility and cytotoxicity could severely delay FDA approval. A desirable alternative would be to fabricate nanostructures from biomaterials such as proteins, peptides, or liposomes, which are already FDA approved. In this article we demonstrate the preparation of protein nanocapsules with both ends sealed using a template-assisted alternate immersion method combined with controlled cleaving. Glucose oxidase nanocapsules with controllable diameter, wall thickness, and length were fabricated and characterized with SEM and TEM. The biochemical activity of glucose oxidase in the form of nanocapsules after processing was confirmed using UV spectrometry. Our future work will explore proteins suitable for drug encapsulation and cellular uptake and will focus on optimizing the cleaving process to gain precise control over the length of the nanocapsules.