Responsivity of the differential-intensity surface plasmon resonance instrument
Responsivity of the differential-intensity surface plasmon resonance instrument
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DOI：
10.1016/j.snb.2016.05.117
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发表时间：
2016-11-01
影响因子：
8.4
通讯作者：
See, Chung W.
中科院分区：
文献类型：
--
作者：
Abayzeed, Sidahmed A.;Smith, Richard J.;See, Chung W.
表面等离子体共振用于生物分析物的微小浓度的灵敏测量和电化学过程的探测。对于强度方法，典型的折射率灵敏度约为10（-6）个折射率单位（RIU）。通过发展差分强度检测方法，提出了更好的灵敏度。该方法依赖于使用平均入射角等于共振角的弱聚焦光束来激发表面等离子体，而反射光使用双电池光电二极管来检测。通过计算其两个单位之间的差来处理双细胞信号，归一化为它们的和。该比率使用用二次函数表示谐振曲线的模型来估计谐振角的偏移。然而，该模型并没有解释参数的影响，如激发光束的角宽度和传感结构的规格对系统的响应。本文提出了一个详细的评价的响应度，使用实验和理论的方法，它可以预测不同参数的影响，铺平了道路，对一个更好的灵敏度的调查和优化系统的设计，为不同的应用。(C)2016年6月，作者。由爱思唯尔公司出版
Surface plasmon resonance is used for the sensitive measurement of minute concentrations of bioanalytes and probing of electrochemical processes. Typical refractive index sensitivity, for the intensity approach, is around 10(-6) refractive index units (RIUs). A better sensitivity has been suggested by developing a differential-intensity detection method. This method relies on the excitation of surface plasmons using a weakly focused beam with the average angle of incidence equal to the resonance angle, while the reflected light is detected using a bi-cell photodiode. The Bi-cell signal is processed by calculating the difference between its two units, normalized to their sum. This ratio estimates the shift in the resonance angle using a model that represents the resonance curve with a quadratic function. However, this model does not explain the effects of parameters such as the angular width of the excitation beam and the specifications of the sensing structure on the system's response. This paper presents a detailed evaluation of the responsivity using experimental and theoretical approaches, which can predict the effect of the different parameters, paving the way towards the investigation of a better sensitivity and the optimization of the system's design for different applications. (C) 2016 The Authors. Published by Elsevier B.V.