Cleavage of the BMP-4 antagonist chordin by zebrafish tolloid
Cleavage of the BMP-4 antagonist chordin by zebrafish tolloid
复制标题
DOI：
10.1126/science.278.5345.1937
复制
发表时间：
1997-12-12
期刊：
影响因子：
56.9
通讯作者：
Strahle, U
中科院分区：
文献类型：
--
作者：
Blader, P;Rastegar, S;Strahle, U
脊椎动物和果蝇胚胎的背腹侧模式需要骨形态发生蛋白（BMP）和BMP活性拮抗剂。果蝇的基因tolloid编码一种类似于BMP-1的金属蛋白酶，这种蛋白酶与脊椎动物BMP-2/4的果蝇同源物decapentapilmal相互作用。研究表明，斑马鱼胚胎过度表达斑马鱼的粪便蛋白同源物，类似于斑马鱼脊索蛋白的功能缺失突变，脊索蛋白是爪蟾BMP-4拮抗剂脊索蛋白的斑马鱼同源物。此外，表达Tolloid的COS细胞可降解Chordin。这些数据表明，Tolloid通过蛋白水解裂解Chordin来对抗Chordin活性，在斑马鱼和果蝇的胚胎发生过程中，Tolloid具有保守的功能。
Dorsoventral patterning of vertebrate and Drosophila embryos requires bone morphogenetic proteins (BMPs) and antagonists of BMP activity. The Drosophila gene tolloid encodes a metalloprotease similar to BMP-1 that interacts genetically with decapentaplegic, the Drosophila homolog of vertebrate BMP-2/4. Zebrafish embryos overexpressing a zebrafish homolog of tolloid were shown to resemble loss-of-function mutations in chordino, the zebrafish homolog of the Xenopus BMP-4 antagonist Chordin. Furthermore, Chordin was degraded by COS cells expressing Tolloid. These data suggest that Tolloid antagonizes Chordin activity by proteolytically cleaving Chordin, A conserved function for zebrafish and Drosophila Tolloid during embryogenesis is proposed.