Time-course of 5-HT6 receptor mRNA expression during memory consolidation and amnesia
Time-course of 5-HT6 receptor mRNA expression during memory consolidation and amnesia
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DOI：
10.1016/j.nlm.2009.08.009
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发表时间：
2010-01-01
影响因子：
2.7
通讯作者：
Meneses, A.
中科院分区：
文献类型：
--
作者：
Huerta-Rivas, A.;Perez-Garcia, G.;Meneses, A.
越来越多的证据表明，5-羟色胺（5-羟色胺）受体（6）（5-HT 6）的拮抗剂可改善记忆和逆转健忘症，尽管对所涉及的机制知之甚少。因此，本文采用RT-PCR方法研究了5-HT_6受体拮抗剂SB-399885和健忘药东莨菪碱或地佐环平对训练和非训练大鼠5-HT_6受体mRNA表达的影响。观察不同时间前额叶皮层、海马和纹状体5-HT 6受体mRNA表达的变化。数据表明，在巴甫洛夫/仪器自动成形任务的记忆是一个渐进的过程与5-HT 6受体的mRNA表达减少在三个结构的检查。SB-399885在48小时改善长期记忆，而毒蕈碱受体拮抗剂东莨菪碱或非竞争性NMDA受体拮抗剂地佐环平在24小时损害长期记忆。在24或48 h，自动塑形训练和SB-399885处理增加了前额叶皮层和纹状体中5-HT 6受体mRNA的表达（最大增加）。东莨菪碱诱导的遗忘抑制5-HT 6受体mRNA的表达，而地佐环平诱导的遗忘没有改变5-HT 6受体mRNA的表达。SB-399885和东莨菪碱或地佐环平能够重建记忆和5-HT 6受体mRNA表达。这些数据证实了先前的记忆证据，更令人感兴趣的是观察到训练、SB-399885和健忘症药物调节了前额叶皮层、海马和纹状体中的5-HT 6受体mRNA表达。进一步研究不同的记忆任务，时间和遗忘模型以及更复杂的控制组可能会提供进一步的线索。(C)2009 Elsevier Inc. All rights reserved.
Growing evidence indicates that antagonists of the 5-hydroxytryptamine (serotonin) receptort(6) (5-HT6) improve memory and reverse amnesia although the mechanisms involved are poorly understood. Hence, in this paper RT-PCR was used to evaluate changes in mRNA expression of 5-HT6 receptor in trained and untrained rats treated with the 5-HT6 receptor antagonist SB-399885 and amnesic drugs scopolamine or dizocilpine. Changes in mRNA expression of 5-HT6 receptor were investigated at different times in prefrontal cortex, hippocampus and striatum. Data indicated that memory in the Pavlovian/instrumental autoshaping task was a progressive process associated to reduced mRNA expression of 5-HT6 receptor in the three structures examined. SB-399885 improved long-term memory at 48 h, while the muscarinic receptor antagonist scopolamine or the non-competitive NMDA receptor antagonist dizocilpine impaired it at 24 h. Autoshaping training and treatment with SB-399885 increased 5-HT6 receptor mRNA expression in (maximum increase) prefrontal cortex and striatum, 24 or 48 h. The scopolamine-induced amnesia suppressed 5-HT6 receptor mRNA expression while the dizocilpine-induced amnesia did not modify 5-HT6 receptor mRNA expression. SB-399885 and scopolamine or dizocilpine were able to reestablish memory and 5-HT6 receptor mRNA expression. These data confirmed previous memory evidence and of more interest is the observation that training, SB-399885 and amnesic drugs modulated 5-HT6 receptor mRNA expression in prefrontal cortex, hippocampus and striatum. Further investigation in different memory tasks, times and amnesia models together with more complex control groups might provide further clues. (C) 2009 Elsevier Inc. All rights reserved.