RNA interference‐mediated silencing of the syntaxin 5 gene induces Golgi fragmentation but capable of transporting vesicles
RNA interference‐mediated silencing of the syntaxin 5 gene induces Golgi fragmentation but capable of transporting vesicles
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DOI：
10.1016/j.febslet.2005.06.053
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发表时间：
2005-08
期刊：
影响因子：
3.5
通讯作者：
Kei Suga;H. Hattori;Ayako Saito;K. Akagawa
中科院分区：
文献类型：
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作者：
Kei Suga;H. Hattori;Ayako Saito;K. Akagawa
研究表明，突触融合蛋白5（Syx 5）参与囊泡转运。我们研究了Syx 5下调对哺乳动物细胞中高尔基体形态和囊泡运输的影响。Syx 5基因的敲除导致高尔基体片段化，而不改变内质网（ER）驻留蛋白、其他高尔基体-SNARE（可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体）和外套蛋白的水平。引人注目的是，Syx 5表达的大幅下降几乎没有影响水泡性口炎病毒G（VSVG）蛋白到质膜的顺行运输。这些结果表明，Syx 5是必需的高尔基体结构的维护，但可能不会发挥主要作用，在运输囊泡携带VSVG之间的ER和高尔基体隔间。
It has been suggested that syntaxin 5 (Syx5) participates in vesicular transport. We examined the effects of Syx5 down-regulation on the morphology of the Golgi apparatus and the transport of vesicles in mammalian cells. Knockdown of the Syx5 gene resulted in Golgi fragmentation without changing the level of endoplasmic reticulum (ER)-resident proteins, other Golgi-SNAREs (soluble N-ethylmaleimide-sensitive factor-attachment protein receptors), and coatmer proteins. Strikingly, a major decrease in Syx5 expression barely affected the anterograde transport of vesicular stomatitis virus G (VSVG) protein to the plasma membrane. These results suggest that Syx5 is required for the maintenance of the Golgi structures but may not play a major role in the transport of vesicles carrying VSVG between the ER and the Golgi compartment.