Magnolol protects channel catfish from Aeromonas hydrophila infection via inhibiting the expression of aerolysin
Magnolol protects channel catfish from Aeromonas hydrophila infection via inhibiting the expression of aerolysin
复制标题
厚朴酚通过抑制气溶素的表达来保护斑点叉尾鮰免受嗜水气单胞菌感染
DOI：
10.1016/j.vetmic.2017.10.005
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发表时间：
2017-11-01
影响因子：
3.3
通讯作者：
Ai, Xiaohui
中科院分区：
文献类型：
--
作者：
Dong, Jing;Ding, Hao;Ai, Xiaohui
嗜水气单胞菌是一种常见的人畜共患病原体，可引起人类和动物，特别是水生动物的多种感染。抗生素已广泛用于治疗嗜水气单胞菌感染，然而，耐药性的发展限制了这些感染的治疗。迫切需要针对这些感染的新药物和策略。气溶素是大多数致病性嗜水气单胞菌分泌的一种成孔毒素，已知它与嗜水气单胞菌感染的发病机制有关。因此，气溶素已被确定为药物发现的潜在靶标。在本文中，我们发现了厚朴酚，一种不含抗 A 的天然化合物。嗜水气单胞菌活性，在低浓度下可通过抑制气溶素编码基因aerA的转录，显着抑制嗜水气单胞菌培养上清液的溶血活性。此外，生存测定表明，厚朴酚可以显着降低斑点叉尾鮰 (Ictalurus punctatus) 嗜水放线菌感染引起的死亡率。总而言之，这些发现提供了一种有效的抗嗜水菌感染的药物。
Aeromonas hydrophila is a common zoonotic pathogen which can cause several infections both in human and animals, particular aquatic animals. Antibiotics have been widely used in the treatment of A. hydrophila infections, however, the development of resistance has limited the treatment for these infections. There is an urgent need for novel agents and strategies against these infections. Aerolysin, a pore-forming toxin secreted by most pathogenic A. hydrophila, is known to contribute to the pathogenesis of A. hydrophila infections. Therefore, aerolysin has been identified as a potential target for drug discovery. In this paper, we found that magnolol, a natural compound without anti-A. hydrophila activity, could significantly inhibit the hemolytic activity of A. hydrophila culture supernatants by inhibiting the transcription of the aerolysin encoding gene aerA at low concentrations. Furthermore, the survival assay showed that magnolol could significantly reduce the mortality induced by A. hydrophila infection in channel catfish (Ictalurus punctatus). Taken together, these findings provide a potent agent against A hydrophila infections.