The effect of synthetic octacalcium phosphate in a collagen scaffold on the osteogenicity of mesenchymal stem cells.
The effect of synthetic octacalcium phosphate in a collagen scaffold on the osteogenicity of mesenchymal stem cells.
复制标题
胶原支架中合成磷酸八钙对间充质干细胞成骨性的影响。
DOI：
10.22203/ecm.v022a10
复制
发表时间：
2011
影响因子：
3.1
通讯作者：
O. Suzuki
中科院分区：
文献类型：
--
作者：
T. Kawai;T. Anada;T. Masuda;Y. Honda;Y. Sakai;Y. Kato;S. Kamakura;S. Echigo;O. Suzuki
虽然合成磷酸八钙（OCP）晶体植入的体内成骨能力的功效可以通过其刺激宿主成骨细胞谱系分化的能力来解释，但OCP支持体内成骨细胞骨再生的直接证据尚未显示。将从4周龄雄性Wistar大鼠长骨分离的间充质干细胞（MSC）在碱性成纤维细胞生长因子（bFGF）存在或不存在的情况下在成骨或维持培养基中预孵育。将OCP/胶原（OCP/Col）或胶原盘用MSC接种，所述MSC已经在含有bFGF的成骨培养基中预孵育，其表现出最高的分化诱导，然后再孵育一天。将磁盘植入12周龄雄性Wistar大鼠的临界大小的颅骨缺损中，并在植入后4周和8周对样本进行放射学、组织学、组织形态学和显微计算机断层扫描（CT）成像分析。OCP/Col·MSCs组快速诱导更多的骨再生，甚至在4周内，与无MSCs的OCP/Col组相比。OCP/Col·MSCs组的骨密度也大于OCP/Col组。Col·MSCs组未表现出明显的成骨作用。这些结果表明，如果在合适的分化条件下预处理细胞，胶原基质中的OCP晶体有效地促进外源引入的成骨细胞启动骨再生。
Although the efficacy of the in vivo osteogenic capabilities of synthetic octacalcium phosphate (OCP) crystal implantation can be explained through its stimulatory capacity for the differentiation of the host osteoblastic cell lineage, direct evidence that OCP supports bone regeneration by osteogenic cells in vivo has not been shown. Mesenchymal stem cells (MSCs) isolated from 4-week-old male Wistar rat long bones were pre-incubated in osteogenic or maintenance medium in the presence or absence of basic fibroblast growth factor (bFGF). OCP/Collagen (OCP/Col) or collagen disks were seeded with MSCs that had been pre-incubated in osteogenic medium containing bFGF, which exhibited the highest differentiation induction, and then incubated for an additional day. The disks were implanted in critical-sized calvaria defects of 12-week-old male Wistar rats and the specimens were analysed radiographically, histologically, histomorphometrically, and by micro-computed tomography (CT) imaging at 4 and 8 weeks after the implantation. The OCP/Col·MSCs group rapidly induced more bone regeneration, even within 4 weeks, compared to the OCP/Col group without MSCs. The bone mineral density of the OCP/Col·MSCs group was also greater than the OCP/Col group. The Col·MSCs group did not exhibit prominent osteogenicity. These results indicate that OCP crystals in a collagen matrix efficiently promote exogenously introduced osteogenic cells to initiate bone regeneration if the cells are pre-treated in a suitable differentiation condition.