Identification and sequence determination of the capsid protein gene of feline calicivirus.
Identification and sequence determination of the capsid protein gene of feline calicivirus.
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DOI：
10.1007/bf01317185
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发表时间：
1992
影响因子：
2.7
通讯作者：
Gaskell RM
中科院分区：
文献类型：
--
作者：
Carter MJ;Milton ID;Turner PC;Meanger J;Bennett M;Gaskell RM
我们从一个包含猫杯状病毒F9株3‘端的cDNA克隆中确定了4380个碱基的序列。我们发现了4个候选开放阅读框，其中3个完整，由245、317和2012个核苷酸组成。第四节继续向5‘端进发。我们已经表达了最大的完整开放阅读框架。Coli.对该抗原免疫的血清与衣壳蛋白及其细胞内前体分子发生特异性反应。对纯化的成熟衣壳蛋白的N末端序列分析证实了这一指定，并确定了前体被切割的位置。
We have determined 4380 bases of the sequence from a cDNA clone containing the 3′ end of feline calicivirus strain F9. We find four candidate open reading frames of which three are complete and comprise 245, 317 and 2012 nucleotides. The fourth continues toward the 5′ end. We have expressed the largest complete open reading frame inE. coli. Sera raised to this antigen react specifically with the capsid protein and its intracellular precursor molecule. N-terminal sequence analysis of purified, mature capsid protein confirms this assignment and has identified the position at which precursor is cleaved.