ANTI-GLOMERULAR BASEMENT-MEMBRANE AUTOANTIBODIES IN THE BROWN NORWAY RAT - DETECTION BY A SOLID-PHASE RADIOIMMUNOASSAY
ANTI-GLOMERULAR BASEMENT-MEMBRANE AUTOANTIBODIES IN THE BROWN NORWAY RAT - DETECTION BY A SOLID-PHASE RADIOIMMUNOASSAY
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DOI：
10.1016/0022-1759(83)90227-2
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发表时间：
1983-01-01
影响因子：
2.2
通讯作者：
LOCKWOOD, CM
中科院分区：
文献类型：
--
作者：
BOWMAN, C;PETERS, DK;LOCKWOOD, CM
本文报道了一种固相放射免疫分析法（RIA），用于检测氯化汞诱发的Brown Norway大鼠肾小球基底膜（GBM）IgG自身抗体。该试验包括将GBM的胶原酶消化物吸附到塑料微量滴定板上，并用亲和纯化的放射性标记兔抗大鼠IgG检测结合抗体。与现有的免疫荧光方法检测抗GBM抗体的比较表明，固相RIA是高度敏感的，允许检测抗体的溶液中低至0.5 ng蛋白/ml。该方法适用于检测血清和肾炎肾洗脱液中的抗GBM抗体。该测定法在GBM、匙孔帽贝抗原和卵清蛋白引起的抑制的竞争性研究中具有特异性。该固相放射免疫分析法具有重现性好、稳定性好、操作简便等特点。
A solid-phase radioimmunoassay (RIA) is described for the detection of IgG autoantibodies to glomerular basement membrane (GBM) induced in the Brown Norway rat by mercuric chloride. The assay involves the adsorption of a collagenase digest of GBM to plastic microtiter plates and detection of bound antibody with affinity purified radiolabeled rabbit anti-rat IgG. Comparison with existing immunofluorescence methods for detection of anti-GBM antibody showed that the solid-phase RIA is highly sensitive, allowing detection of antibody in solutions with as low as 0.5 ng protein/ml. The assay is suitable for detection of anti-GBM antibody both in serum and in eluates from nephritic kidneys. The assay was specific in competitive studies of inhibition brought about by GBM, keyhole limpet antigen and ovalbumin. This solid-phase RIA is reproducible, robust and easy to perform.