Beneficial effect of zeaxanthin on retinal metabolic abnormalities in diabetic rats
Beneficial effect of zeaxanthin on retinal metabolic abnormalities in diabetic rats
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DOI：
10.1167/iovs.07-0764
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发表时间：
2008-04-01
影响因子：
4.4
通讯作者：
Gierhart, Dennis L.
中科院分区：
文献类型：
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作者：
Kowluru, Renu A.;Menon, Bindu;Gierhart, Dennis L.
目的。氧化损伤和生长因子与糖尿病视网膜病变的发病机制有关。最近的研究表明，两种膳食类胡萝卜素，叶黄素和玉米黄质（Zx），特别集中在眼组织中，可能在维持其完整性方面发挥重要作用。本研究旨在评估Zx对糖尿病视网膜氧化损伤和生长因子的潜在保护作用。一组大鼠在诱导糖尿病后立即接受正常的粉状饮食或添加0.02％或0.1％Zx的粉状饮食。年龄匹配的正常大鼠作为对照对象。糖尿病 2 个月时，对视网膜中的氧化应激、血管内皮细胞生长因子 (VEGF) 和细胞间粘附分子 (ICAM)-1 进行定量。补充 Zx 可以防止糖尿病引起的视网膜损伤增加，以及 VEGF 和 ICAM-1 的增加。在Zx治疗的糖尿病大鼠和正常对照大鼠的视网膜中，脂质过氧化物、氧化修饰DNA、电子传递复合物III、硝基酪氨酸和线粒体超氧化物歧化酶的水平相似，并且这些值与没有任何补充的糖尿病大鼠获得的值显着不同。在相同的大鼠中，Zx 还可以阻止糖尿病引起的视网膜 VEGF 和 ICAM-1 的增加。 0.02% 和 0.1% Zx 对糖尿病引起的视网膜异常具有相似的作用，并且这些作用是在没有改善高血糖严重程度的情况下实现的。然而，Zx 给药未能阻止糖尿病引起的视网膜 GSH 水平下降。结论。 Zx 显着抑制糖尿病引起的视网膜氧化损伤以及 VEGF 和粘附分子的升高，所有异常都与糖尿病视网膜病变的发病机制相关。结果表明，补充 Zx 有可能抑制糖尿病患者视网膜病变的发展。
PURPOSE. Oxidative damage and growth factors are implicated in the pathogenesis of retinopathy in diabetes. Recent studies have shown that two dietary carotenoids, lutein and zeaxanthin (Zx), that are specifically concentrated within ocular tissues, may play important roles in maintaining their integrity. This study is to evaluate the potential protective effects of Zx against retinal oxidative damage and growth factors in diabetes.METHODS. A group of rats received normal powdered diet or powdered diet supplemented with 0.02% or 0.1% Zx soon after induction of diabetes. Age-matched normal rats served as control subjects. At 2 months of diabetes, oxidative stress, vascular endothelial cell growth factor ( VEGF), and intercellular adhesion molecule ( ICAM)-1 were quantified in the retina.RESULTS. Zx supplementation prevented diabetes-induced increase in retinal damage, and increases in VEGF and ICAM-1. The levels of lipid peroxide, oxidatively modified DNA, electron transport complex III, nitrotyrosine, and mitochondrial superoxide dismutase were similar in the retinas of Zx-treated diabetic rats and normal control rats, and these values were significantly different from those obtained from diabetic rats without any supplementation. In the same rats, Zx also prevented diabetes-induced increases in retinal VEGF and ICAM-1. Both 0.02% and 0.1% Zx had similar effects on diabetes-induced retinal abnormalities, and these effects were achieved without ameliorating the severity of hyperglycemia. However, Zx administration failed to prevent a diabetes-induced decrease in retinal GSH levels.CONCLUSIONS. Zx significantly inhibits diabetes-induced retinal oxidative damage and elevation in VEGF and adhesion molecule, all abnormalities that are associated with the pathogenesis of diabetic retinopathy. The results suggest that Zx supplementation has the potential to inhibit the development of retinopathy in diabetic patients.