Identification of an inducible factor secreted by pancreatic cancer cell lines that stimulates the production of fucosylated haptoglobin in hepatoma cells
Identification of an inducible factor secreted by pancreatic cancer cell lines that stimulates the production of fucosylated haptoglobin in hepatoma cells
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DOI：
10.1016/j.bbrc.2008.10.061
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发表时间：
2008-12-19
影响因子：
3.1
通讯作者：
Miyoshi, Eiji
中科院分区：
文献类型：
--
作者：
Narisada, Megumi;Kawamoto, Sayuri;Miyoshi, Eiji
岩藻糖基化是最重要的寡糖修饰之一，与癌症和炎症有关。最近，岩藻糖化结合珠蛋白被确定为胰腺癌的一个可能的肿瘤市场。胰腺癌患者血清中岩藻糖化结合珠蛋白升高的分子机制似乎很复杂。我们之前的研究。首页--期刊主要分类--期刊细介绍--期刊题录与文摘--期刊详细文摘内容首页--期刊主要分类--期刊细介绍--期刊题录与文摘--期刊详细文摘内容O.Ishikawa，T Ito，M.Kato，A.Kasahara，S.Kawano，J.Gu，N.Taniguchi，E.Miyoshi，岩藻糖化结合珠蛋白是胰腺癌的新标记物：对岩藻糖化结合珠蛋白的寡糖结构和可能的机制的详细分析，国际癌症118(I)(2006)2803-2808]证明胰腺癌细胞分泌一种诱导肝癌细胞产生结合珠蛋白的因子。在目前的研究中，我们利用IL6的中和抗体发现，在胰腺癌中表达的白介素6(11，6)是诱导结合珠蛋白产生的一个因素。实时定量聚合酶链式反应分析显示IL6处理后岩藻糖化调节基因上调，凝集素酶联免疫吸附试验显示岩藻糖化结合珠蛋白增加。这一途径可能是胰腺癌患者血清中结合珠蛋白升高的可能机制之一。(C)2008 Elsevier Inc.保留所有权利。
Fucosylation is one of the most important oligosaccharide modifications and is involved in cancer and inflammation. Recently, fucosylated haptoglobin was identified as a possible tumor market for pancreatic cancer. The molecular mechanism underlying increases in fucosylated haptoglobin in sera of patients with pancreatic cancer seems to be complicated. Our previous study IN. Okuyama, Y. Ide, M. Nakano, T. Nakagawa, K. Yamanaka. K. Moriwaki, K. Murata, H. Ohigashi, S. Yokoyama, H. Eguchi. O. Ishikawa, T Ito, M. Kato, A. Kasahara, S. Kawano, J. Gu, N. Taniguchi, E. Miyoshi, Fucosylated haptoglobin is a novel marker for pancreatic cancer: a detailed analysis of the oligosaccharide structure and a possible mechanism for fucosylation, Int.J. Cancer 118 (I I) (2006) 2803-2808] demonstrated that pancreatic cancer cells secrete a factor, which induces the production of haptoglobin in hepatoma cells. In the present study, we found that interleukin 6 (11,6) expressed in pancreatic cancer is a factor that induces the haptoglobin production, using a neutralizing antibody for IL6. Real-time PCR analyses revealed the up-regulation of fucosylation regulatory genes after IL6 treatment, resulting increases in fucosylated haptoglobin being revealed by a lectin ELISA. This pathway could be one of the possible mechanisms underlying increases in haptoglobin in sera of patients with pancreatic cancer. (C) 2008 Elsevier Inc. All rights reserved.