Description of the bacterium causing blight of leek as Pseudomonas syringae pv. porri (pv. nov.)
Description of the bacterium causing blight of leek as Pseudomonas syringae pv. porri (pv. nov.)
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DOI：
10.1094/phyto.1998.88.8.844
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发表时间：
1998-08-01
期刊：
影响因子：
3.2
通讯作者：
Gardan, L
中科院分区：
文献类型：
--
作者：
Samson, R;Shafik, H;Gardan, L
通过常规生化方法、血清学反应、数值分类学、DNA-DNA 杂交和冰核活性以及对韭菜和其他寄主植物的致病性，研究了从法国和其他国家的韭菜枯萎病（Allium porrum）中分离的 40 株细菌。将它们与假单胞菌参考菌株进行比较，主要是丁香假单胞菌致病型菌株和丁香假单胞菌 pv. 菌株。从包括洋葱在内的各种寄主植物中分离出丁香菌。韭葱：通过 LOPAT 测试（左聚糖蔗糖酶、氧化酶、果胶酶、精氨酸二水解酶的产生以及对烟草的过敏反应）对丁香假单胞菌 (sensu Late) 进行分类的菌株。韭菜菌株对韭菜具有致病性，并产生与田间观察到的相同的症状。它们是我们研究中唯一可能引起韭菜枯萎病的菌株。因此，我们的结果证明了创建新的Pathovar的合理性。从数值分类上看，韭葱菌株构成了高度同质的DNA群和离散的苯酚，属于O血清群FOR。 P. syringae pv. 的名称。 porri 被提议用于引起韭菜枯萎病的细菌。提出了常规鉴定的标准并讨论了分类地位。
Forty bacterial strains isolated from leek blight (Allium porrum) in France and other countries were studied by conventional biochemical methods, serological reactions, numerical taxonomy, DNA-DNA hybridization, and ice nucleation activity, as well as by pathogenicity on leek and other host plants. They were compared with reference strains of Pseudomonas, mainly pathotype strains of P. syringae pathovars and strains of P. syringae pv. syringae isolated from various host plants including onions. Leek:strains sorted with P. syringae species (sensu late) by LOPAT tests (production of levan-sucrase, oxidase, pectinase, arginine dihydrolase, and hypersensitive reaction on tobacco). Leek strains were pathogenic to leek and produced symptoms identical to those observed in the field. They were the only strains in our study that could cause blight of leek. Thus, our results justify the creation of a new pathovar. Leek strains constituted a highly homogeneous DNA group and a discrete phenon by numerical taxonomy, and they belonged to O-serogroup FOR. The name of P. syringae pv. porri is proposed for the bacterium causing leek blight. Criteria for routine identification are presented and taxonomic status is discussed.