Interleukin 17-producing γδ T cells increased in patients with active pulmonary tuberculosis
Interleukin 17-producing γδ T cells increased in patients with active pulmonary tuberculosis
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DOI：
10.1038/cmi.2008.25
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发表时间：
2008-06-01
影响因子：
24.1
通讯作者：
Li, Baiqing
中科院分区：
文献类型：
--
作者：
Peng, Meiyu;Wang, Zhaohua;Li, Baiqing
虽然已经知道γ δ T细胞在对结核分枝杆菌感染的免疫应答中可能起重要作用（M. tb），γ δ T细胞参与对结核病（TB）的先天性和/或获得性免疫的机制尚未完全阐明。在本研究中，27例活动性肺结核患者和16名健康供体（HD）进行。我们发现，在TB患者和HD患者之间，淋巴细胞中IL-17产生细胞的比例相似，而TB患者外周血中γ δ T细胞在IL-17产生细胞中的比例（59.2%）和γ δ T细胞中IL-17产生细胞的比例（19.4%）与HD患者相比（分别为43.9%和7.7%）显著增加。此外，结核病患者中产生干扰素γ的γ δ T细胞比例明显低于HD患者。在用M. tb热处理抗原（M. tb-HAg），从HD和TB患者中产生较少的IL-17产生γ δ T细胞，而与HD相比，TB患者中产生IFN-γ的γ δ T细胞增加。我们在结核病人和健康人中的发现与其他小鼠研究结果一致，即产生IL-17的γ δ T细胞是BCG感染小鼠模型中IL-17的主要来源，提示γ δ T细胞可能参与肺结核病人结核性肉芽肿的形成，但需要进一步鉴定。
Although it has been known that gamma delta T cells may play an important role in the immune response to infection of Mycobacterium tuberculosis (M. tb), the mechanisms by which the gamma delta T cells participate in the innate and/or acquired immunity to tuberculosis (TB) have not been full elucidated. In the present study, 27 patients with active pulmonary TB and 16 healthy donors (HD) were performed. We found that proportion of IL-17-producing cells among lymphocyte was similar between TB patients and HD, whereas the proportions of gamma delta T cells in IL-17-producing cells (59.2%) and IL-17-producing cells in gamma delta T cells (19.4%) in peripheral blood were markedly increased in TB patients when compared to those in HD (43.9% and 7.7%, respectively). In addition, the proportions of IFN-gamma-producing gamma delta T cells in TB patients were obviously lower than that in HD. Upon re-stimulated with M. tb heat-treated antigen (M. tb-HAg) in vitro, fewer IL-17-producing gamma delta T cells were generated from HD and TB patients, whereas IFN-gamma-producing gamma delta T cells were increased in TB patients compared to that in HD. Our findings in TB patients and healthy human were consistent with other murine investigation that the IL-17-producing gamma delta T cells were main source of IL-17 in mouse model of BCG infection, suggesting that gamma delta T cells might be involved in the formation of tubercular granuloma in pulmonary TB patients, but need further identification.