3-Hydroxyphthalic Anhydride-Modified Chicken Ovalbumin Exhibits Potent and Broad Anti-HIV-1 Activity: a Potential Microbicide for Preventing Sexual Transmission of HIV-1
3-Hydroxyphthalic Anhydride-Modified Chicken Ovalbumin Exhibits Potent and Broad Anti-HIV-1 Activity: a Potential Microbicide for Preventing Sexual Transmission of HIV-1
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3-羟基邻苯二甲酸酐修饰的鸡卵清蛋白表现出有效且广泛的抗 HIV-1 活性：一种预防 HIV-1 性传播的潜在杀菌剂
DOI：
10.1128/aac.01046-09
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发表时间：
2010-05-01
影响因子：
4.9
通讯作者：
Liu, Shuwen
中科院分区：
文献类型：
--
作者：
Li, Lin;He, Lili;Liu, Shuwen
异性性传播是妇女感染人体免疫机能丧失病毒（艾滋病毒）/艾滋病的主要途径。因此，迫切需要开发妇女控制的局部杀微生物剂，以预防艾滋病毒的性传播。在这里，我们报告，3-羟基邻苯二甲酰苯胺修饰的鸡卵清蛋白（HP-OVA）表现出强大的抗病毒活性，对广谱的人类免疫缺陷病毒1型（HIV-1）分离株与不同的基因型和生物型。其抗病毒活性与OVA中化学修饰和未修饰的赖氨酸和赖氨酸的百分比相关。HP-OVA通过多种作用机制抑制HIV-1融合和进入，包括（i）阻断gp 120与CD 4的结合和（ii）干扰gp 41六螺旋束的形成。由于OVA的广泛可用性和已确定的安全性，HP-OVA具有良好的潜力被开发为一种有效、安全和负担得起的杀微生物剂，用于预防HIV性传播。
Heterosexual transmission is the primary route by which women acquire human immunodeficiency virus (HIV)/AIDS. Thus, development of woman-controlled topical microbicides for prevention of sexual transmission of HIV is urgently needed. Here we report that 3-hydroxyphthalic anhydride-modified chicken ovalbumin (HP-OVA) exhibits potent antiviral activity against a broad spectrum of human immunodeficiency virus type 1 (HIV-1) isolates with different genotypes and biotypes. Its antiviral activity is correlated with the percentages of the chemically modified and unmodified lysines and arginines in OVA. HP-OVA inhibits HIV-1 fusion and entry through multiple mechanisms of action, including (i) blocking gp120 binding to CD4 and (ii) interfering with gp41 six-helix bundle formation. Because of the widespread availability and established safety profile of OVA, HP-OVA has good potential to be developed as an effective, safe, and affordable microbicide for prevention of HIV sexual transmission.