Virulence-associated protein characterisation of Rhodococcus equi isolated from bovine lymph nodes
Virulence-associated protein characterisation of Rhodococcus equi isolated from bovine lymph nodes
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DOI：
10.1016/s0378-1135(00)00297-2
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发表时间：
2001-02-12
影响因子：
3.3
通讯作者：
Takai, S
中科院分区：
文献类型：
--
作者：
Flynn, O;Quigley, F;Takai, S
马红球菌在牛中的致病性较低，但偶尔会引起淋巴结肉芽肿，在屠宰场尸检时检测到，必须与结核性肉芽肿区分开来。1997年和1998年期间，在爱尔兰共和国屠宰场进行尸检的总计3，263.622头牛中，在6719头牛中检测到淋巴结病变。对所有病变进行组织学检查，主要目的是识别患有结核病的动物。共1122个病灶在血琼脂和含有丙酮酸盐的Stonebrinks和Lowenstein-Jensen培养基上培养，因为组织学结果难以解释或提示R。马传染病R.从264个病灶中分离出马。几乎所有的R。马肉芽肿局限于单个淋巴结，主要存在于咽后、支气管和纵隔淋巴结。R.与母牛相比，在阉牛和小母牛中检测到的马肉芽肿的病变比例显著更高。在尸检的330万头牛的总群体中，患病率为0.008%。15-17 kDa的抗原，在这种生物体的毒力，和20 kDa的抗原，与中等毒力，未检测到分离物从146头牛，免疫印迹分析。用于检测编码15-17 kDa抗原的质粒基因的PCR试验也对来自这146只动物的分离株呈阴性。对30株菌株进行了检测，未检出质粒。(C)2001 Elsevier Science B. V.保留所有权利。
Rhodococcus equi has a low pathogenicity in cattle, but it occasionally causes lymph node granulomas, which are detected at abattoir post mortem inspection, and must be distinguished from tuberculous granulomas. Lymph node lesions were detected in 6719 cattle, from a total of 3,263.622 cattle examined post mortem in abattoirs, in the Republic of Ireland, during 1997 and 1998. Histological examination was performed on all lesions, principally for the purpose of identifying animals with tuberculosis. A total of 1122 of the lesions were cultured on blood agar and on Stonebrinks and Lowenstein-Jensen medium containing pyruvate, because the histological findings were difficult to interpret or were suggestive of R. equi infection. R. equi was isolated from 264 lesions. Almost all of the R. equi granulomas were confined to a single lymph node, and were present predominantly in the retropharyngeal, bronchial and mediastinal lymph nodes. R. equi granulomas were present in a significantly higher proportion of the lesions detected in steers and heifers compared to cows. The prevalence in the total population of 3.3 million cattle examined post mortem was 0.008%. The 15-17 kDa antigens, associated with virulence in this organism, and the 20 kDa antigen, associated with intermediate virulence, were not detected in isolates from 146 cattle, analysed by immunoblot assays. A PCR assay to detect the plasmid gene encoding the 15-17 kDa antigens was also negative for isolates from these 146 animals. Plasmids were not detected in 30 isolates which were examined. (C) 2001 Elsevier Science B.V. All rights reserved.