Cranberry phytochemicals: Isolation, structure elucidation, and their antiproliferative and antioxidant activities
Cranberry phytochemicals: Isolation, structure elucidation, and their antiproliferative and antioxidant activities
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DOI：
10.1021/jf061058l
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发表时间：
2006-09-20
影响因子：
6.1
通讯作者：
Liu, Rui Hai
中科院分区：
文献类型：
--
作者：
He, Xiangjiu;Liu, Rui Hai
蔓越莓的生物活性导向分级用于确定生物活性成分的化学身份。采用梯度溶剂分离、硅胶柱和ODS柱以及制备型RP-HPLC分离得到20个化合物。通过HR-MS、1D和2D NMR以及X-射线衍射分析确定了它们的化学结构。评价了分离的化合物对HepG 2人肝癌和MCF-7人乳腺癌细胞的抗增殖活性。在分离的化合物中，熊果酸、槲皮素和3，5，7，3 '，4'-五羟基黄酮醇-3-O-β-D-吡喃葡萄糖苷显示出对HepG 2细胞生长的有效抗增殖活性，其EC 50值分别为87.4 +/- 2.7、40.9 +/- 1.1和49.2 +/- 4.9 μ M。熊果酸、槲皮素和3，5，7，3 '，4'-五羟基黄酮醇-3-O-β-D-吡喃葡萄糖苷对MCF-7细胞的增殖表现出有效的抑制活性，其EC 50值分别为11.7 +/- 0.1、137.5 +/- 2.6和23.9 +/- 3.9 μ M。槲皮素、3，5，7，3 '，4'-五羟基黄酮醇-3-O-β-D-吡喃葡萄糖苷、3，5，7，3 '，4 '-五羟基黄酮醇-3-O-β-D-吡喃半乳糖苷和3，5，7，3 '，4'-五羟基黄酮醇-3-O-α-L-阿拉伯呋喃糖苷显示出有效的抗氧化活性，EC 50值约为10 μ M。这些结果表明蔓越莓植物化学提取物具有有效的抗氧化和抗增殖活性。
Bioactivity-guided fractionation of cranberries was used to determine the chemical identity of bioactive constituents. Twenty compounds were isolated using gradient solvent fractionation, silica gel and ODS columns, and preparative RP-HPLC. Their chemical structures were identified using HR-MS, 1D and 2D NMR, and X-ray diffraction analysis. Antiproliferative activities of isolated compounds against HepG2 human liver cancer and MCF-7 human breast cancer cells were evaluated. Among the compounds isolated, ursolic acid, quercetin, and 3,5,7,3 ',4 '-pentahydroxyflavonol-3-O-beta-D-glucopyranoside showed potent antiproliferative activities against HepG2 cell growth, with EC50 values of 87.4 +/- 2.7, 40.9 +/- 1.1, and 49.2 +/- 4.9 mu M, respectively. Ursolic acid, quercetin, and 3,5,7,3 ',4 '-pentahydroxyflavonol-3-O-beta-D-glucopyranoside showed potent inhibitory activity toward the proliferation of MCF-7 cells, with EC50 values of 11.7 +/- 0.1, 137.5 +/- 2.6, and 23.9 +/- 3.9 mu M, respectively. Quercetin, 3,5,7,3 ',4 '-pentahydroxyflavonol-3-O-beta-D-glucopyranoside, 3,5,7,3,' 4 '-pentahydroxyflavonol3- O-beta-D-galactopyranoside, and 3,5,7,3 ',4 '-pentahydroxyflavonol-3-O-alpha-L-arabinofuranoside showed potent antioxidant activities, with EC50 values of approximate to 10 mu M. These results showed cranberry phytochemical extracts have potent antioxidant and antiproliferative activities.