Panax notoginseng saponin attenuates the hypoxic–ischaemic injury in neonatal rats by regulating the expression of neurotrophin factors
Panax notoginseng saponin attenuates the hypoxic–ischaemic injury in neonatal rats by regulating the expression of neurotrophin factors
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DOI：
10.1111/ejn.15428
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发表时间：
2021-08
影响因子：
3.4
通讯作者：
Jin Huang;Ya-Xin Tan;Lu-Lu Xue-Lu;Ruo‐Lan Du;Jun‐Jie Chen;Li Chen;Ting-ting Li;Xue Bai;Si-Jin Yang;Liulin Xiong;Tinghua Wang
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作者：
Jin Huang;Ya-Xin Tan;Lu-Lu Xue-Lu;Ruo‐Lan Du;Jun‐Jie Chen;Li Chen;Ting-ting Li;Xue Bai;Si-Jin Yang;Liulin Xiong;Tinghua Wang
新生儿缺氧缺血性损伤是新生儿窒息的严重并发症，是新生儿急性死亡和慢性神经损伤的主要原因，目前尚缺乏有效的治疗方法来改善患者的预后。我们在本研究中报道三七总皂苷(PNS)可能为HI提供一种治疗选择。采用新生SD大鼠建立Hi模型，给予不同剂量的三七总皂甙，每日1次，连续7d。通过组织学染色和行为学评价，了解三七总皂苷治疗后的病理变化和神经行为变化。结果发现，三七总皂甙能显著缩小新生大鼠脑梗塞体积，提高自主活动能力，尤其是高剂量组更明显。三七总皂苷40 mg/kg可减轻神经元损伤，抑制神经元凋亡，抑制星形胶质细胞反应。此外，经40 mg/kg三七总皂甙治疗后，远期认知功能和运动功能也有改善。重要的是，三七总皂甙治疗提高了HI大鼠大脑皮层和海马区BDNF和TrkB的水平，但降低了p75NTR的表达。三七总皂苷的治疗作用可能与三七总皂苷激活BDNF/TrkB信号和抑制p75NTR表达有关，为减轻缺氧缺血性脑损伤提供了一种新的治疗方法。
Neonatal hypoxic–ischaemic (HI) injury is a serious complication of neonatal asphyxia and the leading cause of neonatal acute death and chronic neurological injury, and the effective therapeutic method is lacking to improve patients' outcomes. We reported in this study that panax notoginseng saponin (PNS) may provide a treatment option for HI. HI model was established using neonatal Sprague–Dawley rats and then intraperitoneally injected with different dosage of PNS, once a day for 7 days. Histological staining and behavioural evaluations were performed to elucidate the pathological changes and neurobehavioural variation after PNS treatment. We found PNS administration significantly reduced the infarct volume of brain tissues and improved the autonomous activities of neonatal rats, especially with higher dosage. PNS treatment at 40 mg/kg reduced neuronal damage, suppressed neuronal apoptosis and depressed astroglial reactive response. Moreover, the long‐term cognitive and motor functions were also improved after PNS treatment at 40 mg/kg. Importantly, PNS treatment elevated the levels of BDNF and TrkB but decreased the expression of p75NTR both in the cortex and hippocampus of HI rats. The therapeutic efficacy of PNS might be correlated with PNS‐activated BDNF/TrkB signalling and inactivation of p75NTR expression, providing a novel potential therapy for alleviating HI injury.