Microamperometric estimation of photosynthesis inhibition in a single algal protoplast.
Microamperometric estimation of photosynthesis inhibition in a single algal protoplast.
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单个藻类原生质体中光合作用抑制的微电流估计。
DOI：
10.1006/bbrc.1993.2616
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发表时间：
1993
影响因子：
3.1
通讯作者：
I. Uchida
中科院分区：
文献类型：
--
作者：
T. Matsue;S. Koike;I. Uchida
利用插入单藻原生质体的超微电极对光辐射诱导的细胞内氧还原电流的响应，研究了某些化学物质对单藻光合作用的抑制作用。电子传递抑制剂3-（3，4-二氯苯基）-1，1-二甲基脲（DCMU）的加入，使光照射后立即出现的电流峰降低。根据响应模式的变化确定pI 50值（50%抑制所需浓度的负十进对数），发现DCMU的pI 50值为6.9。生长素类除草剂2，4-二氯苯氧乙酸（2，4-D）在有效浓度范围内对光合作用无影响。质子载体2，4-二硝基苯酚（DNP）在光照下加速了氧的产生。
The single-cell level inhibition of photosynthesis by some chemicals was investigated from the light-irradiation induced response of intracellular oxygen reduction current at an ultramicroelectrode inserted into a single algal protoplast. The addition of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), an electron-transport inhibitor, decreased a current peak appeared immediately after the light irradiation. The pI50 value (the negative decadic logarithm of the concentration needed for 50% inhibition) was determined from the change in response patterns and found to be 6.9 for DCMU. 2,4-Dichlorophenoxyacetic acid (2,4-D), an auxin herbicide, showed no effect on photosynthesis in the effective concentration range. 2,4-Dinitrophenol (DNP), a proton carrier, accelerated the oxygen production upon light irradiation.