Structural studies of the carbohydrate moiety of human antithrombin III.
Structural studies of the carbohydrate moiety of human antithrombin III.
复制标题
人抗凝血酶 III 碳水化合物部分的结构研究。
DOI：
10.1016/0003-9861(80)90199-x
复制
发表时间：
1980
影响因子：
3.9
通讯作者：
A. Kobata
中科院分区：
文献类型：
--
作者：
T. Mizuochi;J. Fujii;K. Kurachi;A. Kobata
人抗凝血酶III在一个分子中含有四个天冬酰胺连接的糖链。糖链通过联氨分解、Byn-乙酰化和NaB3H4还原从多肽部分以放射性低聚糖的形式定量释放。所有这样得到的寡糖都含有N-乙酰神经氨酸。通过唾液酸酶处理，所有酸性寡糖都能释放出相同的中性二十三醇。经酶切鉴定和甲基化分析，其结构分别为NeuAcα2→6Galβ1→4GlcNAcβ1→2manα1→6-(NeuAcα2→6Galβ1→4GlcNAcβ1→2manα1→3)Manβ1→4GlcNAcβ1→4GlcNAcβ1→4GlcNAcβ1→2manα1→6(NeuAcα2→6Galβ1→4GlcNAcβ1→2Manα1→4GlcNAcβ1→2Manα1穆勒4GlcNAc SR.1穆勒4GlcNAc)和NeuAc 4GlcNAc SR.1穆勒4GlcNAc SR.1β4GlcNAcα1 L.3 4GlcNAcα1→2GlcNAcβ1→4GlcNAcβ1→2manα1→6(NeuAcα2→6 Galβ1→4GlcNAc忧伤1 ant 4GlcNAc SR.1β4GlcNAcβ1 L.3 4GlcNAcβ1 L.3 4GlcNAc 4cc)Man
Human antithrombin III contains four asparagine-linked sugar chains in one molecule. The sugar chains were quantitatively released as radioactive oligosaccharides from the polypeptide portion by hydrazinolysis followed byN-acetylation and NaB3H4reduction. All of the oligosaccharides, thus obtained, containN-acetylneuraminic acid. A same neutral nonaitol was released from all acidic oligosaccharides by sialidase treatment. By combination of the sequential exoglycosidase digestion and methylation analysis, their structures were elucidated as NeuAcα2 → 6Galβ1 → 4GlcNAcβ1 → 2Manα1 → 6-(NeuAcα2 → 6Galβ1 → 4GlcNAcβ1 → 2Manα1 → 3)Manβ1 → 4GlcNAcβ1 → 4GlcNAc, Galβ1 → 4GlcNAcβ1 → 2Manα1 → 6(NeuAcα2 → 6Galβ1 → 4GlcNAcβ1 → 2Manαl → 3)Manβ1 → 4GlcNAcβ1 → 4GlcNAc, and NeuAcα2 → 6Galβ1 → 4GlcNAcβ1 → 2Manα1 → 6(Galβ1 → 4GlcNAcβ1 → 2Manα1 → 3)Manβ1 → 4GlcNAcβ1 → 4GlcNAc.