Effect of hypoxia on expression of selected proteins involved in regulation of apoptotic activity in striatum of newborn piglets.
Effect of hypoxia on expression of selected proteins involved in regulation of apoptotic activity in striatum of newborn piglets.
复制标题
DOI：
10.1007/s11064-010-0394-x
复制
发表时间：
2011-05
影响因子：
4.4
通讯作者：
Pastuszko, A.
中科院分区：
文献类型：
--
作者：
Mammen, A.;Kubin, J.;Greeley, W. J.;Schears, G. J.;Pastuszko, P.;Wilson, D. F.;Pastuszko, A.
在经受精确控制的1小时低氧损伤随后0、2和4小时恢复的仔猪的纹状体中测量抑制或促进凋亡性细胞死亡的所选神经调节蛋白的水平，并与假手术动物进行比较。抗凋亡蛋白：存活素在0（157%，P = 0.031）和4 h（171%，P = 0.033），在0（138%，P = 0.028）和4 h（143%，P = 0.007），VEGF在4 h（185%，P = 0.019）和HSP 27在2 h（144%，P = 0.05）和4 h（143%，P = 0.05）。促凋亡蛋白caspase-1和caspase-7在4h分别增加135%（P = 0.05）和129%（P = 0.038）。Bim在4h后增加（115%，P = 0.028），凋亡诱导因子在2h后增加（127%，P = 0.048），钙蛋白酶在4h后增加（143%，P = 0.04）。缺氧引起抗凋亡蛋白和促凋亡蛋白水平的增加。它们的相对活性决定了细胞损伤和神经元缺陷的结果。
The levels of selected neuroregulatory proteins that inhibit or promote apoptotic cell death were measured in the striatum of piglets subjected to precisely controlled 1 h hypoxic insult followed by 0, 2 and 4 h recovery and compared to sham operated animals. The anti-apoptotic proteins: there were increases in Survivin at 0 (157%, P = 0.031) and 4 h (171%, P = 0.033), in Bcl-XL at 0 (138%, P = 0.028) and 4 h (143%, P = 0.007), in VEGF at 4 h (185%, P = 0.019) and Hsp27 at 2 h (144%, P = 0.05) and 4 h (143%, P = 0.05). The pro-apoptotic proteins: caspases-1 and 7 increased at 4 h (135%, P = 0.05) and (129%, P = 0.038), respectively. Bim increased after 4 h (115%, P = 0.028), Apoptosis Inducing Factor after 2 h (127%, P = 0.048) and Calpain after 4 h (143% of control, P = 0.04). Hypoxia causes increase in levels of both anti- and pro-apoptotic proteins. Their relative activity determines the outcome in terms of cell damage and neuronal deficit.
登录
查看更多内容
影响因子：
20.3
作者：
Dumont, C;Dürrbach, A;Senik, A
通讯作者：
Senik, A
影响因子：
1.7
作者：
Aly, Hany;Hassanein, Sahar;Atiea, Wael
通讯作者：
Atiea, Wael
影响因子：
5.3
作者：
Cheung, ECC;Melanson-Drapeau, L;Slack, RS
通讯作者：
Slack, RS
DOI：
10.1073/pnas.2336254100
发表时间：
2003-12-09
影响因子：
11.1
作者：
Kuan, CY;Whitmarsh, AJ;Rakic, P
通讯作者：
Rakic, P
影响因子：
15.9
作者：
Cheng, Y;Deshmukh, M;Holtzman, DM
通讯作者：
Holtzman, DM