Appearance of glucocorticoid receptors in the inner ear of the mouse during development
Appearance of glucocorticoid receptors in the inner ear of the mouse during development
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DOI：
10.3109/00016489609137913
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发表时间：
1996-09-01
影响因子：
1.4
通讯作者：
Hultcrantz, M
中科院分区：
文献类型：
--
作者：
Erichsen, S;BaggerSjoback, D;Hultcrantz, M
CBA小鼠在不同的发育年龄处死：胚胎第13天（E13）、E14、E16、E19、E20和出生后第1天（P1）、P2、P3、P4、P6、P8、P10、P14、P16、P18、P20和P30。快速取出颞骨并深度冷冻，以制备冷冻切片，用于用抗糖皮质激素受体的多克隆抗体进行免疫组织化学染色。使用抗生物素蛋白-生物素、ABC 方法来可视化结合。分析了内耳的前庭和耳蜗区域。 E19 处可见壶腹嵴、椭圆囊和耳蜗管的微弱染色；并且染色在 P1 处变得清晰可见。在产后第 2-5 天发现标记减少，此后再次注意到染色增加，直到产后第 14 天观察到成人模式。发育过程中内耳中糖皮质激素受体的出现并不遵循线性曲线。此外，标记模式可能表明糖皮质激素受体对胚胎成熟本身的影响以及在成人耳朵中的功能作用。
CBA mice were sacrificed at different ages of development: at embryonic day 13 (E13), E14, E16, E19, E20 and postnatal day 1 (P1), P2, P3, P4, P6, P8, P10, P14, P16, P18, P20 and P30. The temporal bones were quickly removed and deep frozen in order to prepare cryosections for immunohistochemical staining with polyclonal antibodies against glucocorticoid receptors. The avidin-biotin, ABC-method was used to visualize binding. Both the vestibular and the cochlear regions of the inner ear were analysed. A faint staining of the crista ampullaris, the utricle and the cochlear duct was seen at E19; and staining became clearly visible at P1. A decrease in labelling was found at day 2-5 post partum whereafter an increased staining was again noticed until postnatal day 14 when an adult pattern was observed. The appearance of glucocorticoid receptors in the inner ear during development does not follow a linear curve. Further, the labelling pattern may indicate an impact of glucocorticoid receptors on the embryologic maturation itself as well as a functional role in the adult ear.