Salivary electrolytes, renin, and aldosterone during sodium loading and depletion.
Salivary electrolytes, renin, and aldosterone during sodium loading and depletion.
复制标题
钠负荷和耗竭期间的唾液电解质、肾素和醛固酮。
DOI：
10.1152/jappl.1973.35.3.322
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发表时间：
1973
影响因子：
3.3
通讯作者：
J. Laragh
中科院分区：
文献类型：
--
作者：
S. Wotman;L. Baer;I. Mandel;J. Laragh
方法6名正常受试者在知情同意的情况下，在纽约市长老会医院的代谢科接受恒定的钠饮食。受试者包括三名女性和三名男性，年龄在18-24岁之间，健康状况良好，血压正常：他们在研究之前或期间没有服用任何药物。在每5天期间，他们接受每天含有10mEq钠的饮食。第一个5天期间不补充盐，将被称为低钠饮食。在第二个5天期间，每天给予5g非肠道包衣氯化钠胶囊，以达到97mEq/d的恒定钠摄入量。这将被称为正常的钠饮食。在第三个5天期间，每天给予10g氯化钠胶囊，以达到恒定的185mEq/d钠摄入量。这一时期被称为高钠时期。每天获取24小时的尿样，并分析钠和钾。在每个周期的第5天测定血浆肾素活性和醛固酮排泄率。每天同一时间采集腮腺、颌下腺和全唾液。用改良的Lashley杯子收集腮腺唾液，用Block-Brottman收集器(2)对每个受试者进行改良，获得颌下唾液。以ZO设定的间隔在舌头上擦拭2%柠檬酸以刺激分泌。通过咀嚼橡皮筋并将唾液排入收集管来刺激整个唾液。记录流率，并分析唾液样本中的钠、钾、钙和总蛋白。采用火焰分光光度法测定尿和血清中的钠、钾。血浆肾素活性测定采用放射免疫法测定血浆中血管紧张素I含量。
METHODSSix normal subjects, with their informed consent, were placed on constant sodium diets on the Metabolism Unit of the Presbyterian Hospital in New York City. The subjects consisted of three women and three men, ages 18-24 years, in excellent health and with normal blood pressures: they were on no medication before or during the study periods. During each 5-day period they received a diet containing 10 mEq sodium/day. The first 5-day period consisted of no salt supplement and will be referred to as the low-sodium diet. During the second 5-day period, 5 g of nonenteric coated sodium chloride capsules were administered each day to achieve a constant 97 mEq sodium intake/day. This will be referred to as the normal sodium diet. In the third 5-day period, 10 g of sodium chloride capsules each day were administered to achieve a constant 185 mEq sodium intake/day. This period is referred to as the high-sodium period. Twenty-four-hour urine specimens were obtained daily and analyzed for sodium and potassium. Plasma renin activity and aldosterone excretion rates were determined on the 5th day of each period. Parotid, submaxillary, and whole saliva were collected daily at the same time each day. Parotid saliva was collected with the use of a modified Lashley cup and submaxillary saliva was obtained with the Block-Brottman collector (2) modified for each subject. Secretion was stimulated by swabbing 2% citric acid on the tongue at ZO-set intervals. Whole saliva was stimulated by chewing a rubber band and expectorating into a collecting tube. Flow rates were recorded and saliva samples were analyzed for sodium, potassium, calcium, and total protein. Sodium and potassium in urine and serum were determined by flame photometry. Plasma renin activity was determined by radioimmunoassay of the angiotensin I produced during