Differential activation and conserved vegetative cell-specific activity of a late pollen promoter in species with bicellular and tricellular pollen
Differential activation and conserved vegetative cell-specific activity of a late pollen promoter in species with bicellular and tricellular pollen
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双细胞和三细胞花粉物种中晚期花粉启动子的差异激活和保守的营养细胞特异性活性
DOI：
10.1046/j.1365-313x.1994.05040543.x
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发表时间：
1994
期刊：
影响因子：
7.2
通讯作者：
D. Twell
中科院分区：
文献类型：
--
作者：
C. Eady;K. Lindsey;D. Twell
总结
为了研究番茄晚花粉基因lat 52启动子在二细胞和三细胞花粉物种中的保守性，在烟草和拟南芥中分析了lat 52启动子的激活和发育调控。通过荧光分析和GUS活性的组织化学分析以及RNA分析，详细研究了含有与β-葡萄糖醛酸酶（gus）基因融合的lat 52启动子的转基因植物中的启动子活性。这些研究表明，在转基因A. lat 52启动子在单核小孢子后期小孢子有丝分裂前被激活，而在转基因拟南芥中，lat 52启动子在单核小孢子有丝分裂前被激活。在年轻的双细胞花粉粒中，在小孢子有丝分裂之后立即检测到tabacumlat 52启动子活性。因此，lat 52启动子的精确激活并不严格依赖于A. thaliana.尽管有这种时间上的差异，lat 52启动子活性模式在营养细胞成熟过程中表现出非常相似的累积模式的活动，在这两个物种，这是与一个类似的积累gus转录和孢子蛋白质含量。此外，lat 52启动子核靶向β-葡萄糖醛酸酶融合蛋白的表达使lat 52启动子的活性在花粉发育过程中特异定位于营养细胞。thaliana.
Summary
The activation and developmental regulation of the promoter of the tomato late pollen gene lat52 was analysed in Nicotiana tabacum and Arabidopsis thaliana to investigate the conservation of regulatory mechanisms in species with bicellular and tricellular pollen. Promoter activity in transgenic plants containing the lat52 promoter fused to the β-glucuronidase (gus) gene was studied in detail throughout pollen development by fluorimetric and histochemical analysis of GUS activity, and by RNA analysis. These studies showed that in transgenic A. thaliana the lat52 promoter was activated in late uninucleate microspores immediately prior to microspore mitosis, whereas in transgenic N. tabacum lat52 promoter activity was first detectable immediately following microspore mitosis in young bicellular pollen grains. Thus, the precise activation of the lat52 promoter was not strictly dependent on passage through microspore mitosis in A. thaliana. Despite this temporal difference, the pattern of lat52 promoter activity during vegetative cell maturation showed a very similar cumulative pattern of activity in both species, which was correlated with a similar accumulation of gus transcript and spore protein content. Furthermore, the expression of a lat52 promoter directed nuclear-targeted β-glucuronidase fusion protein allowed lat52 promoter activity to be localized specifically to the vegetative cell during pollen development in A. thaliana.