Fabrication of three-dimensional nano, micro and micro/nano scaffolds of porous poly(lactic acid) by electrospinning and comparison of cell infiltration by Z-stacking/three-dimensional projection technique.
Fabrication of three-dimensional nano, micro and micro/nano scaffolds of porous poly(lactic acid) by electrospinning and comparison of cell infiltration by Z-stacking/three-dimensional projection technique.
复制标题
静电纺丝法制备多孔聚乳酸三维纳米、微米和微/纳米支架，并通过Z-堆叠/三维投影技术比较细胞浸润。
DOI：
10.1049/iet-nbt.2011.0028
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发表时间：
2012
影响因子：
2.3
通讯作者：
R. Jayakumar
中科院分区：
文献类型：
--
作者：
K. Shalumon;K. Chennazhi;H. Tamura;K. Kawahara;S. Nair;R. Jayakumar
电纺细胞外基质（ECM）模拟纳米纤维支架用于组织工程的使用受到细胞浸润不良的限制。作者假设，通过使用纳米纤维与微米级纤维结合的分层结构，可以增强支架中的细胞渗透，同时保留整体支架结构。为了评估这一点，我们制造了具有纳米级，微米级和组合的微/纳米结构的静电纺丝多孔聚乳酸（PLA）支架，并详细评估了结构特征和生物反应。虽然生物活性介于纳米纤维和微纤维支架的生物活性之间，但本研究的独特结果是微/纳米组合纤维支架显示出比纳米纤维支架改善的细胞浸润和分布。尽管在纳米纤维支架的情况下发现细胞排列在支架周边，但微/纳米支架具有分散在整个支架中的细胞。此外，正如预期的那样，加入羟基磷灰石纳米颗粒（nHAp）改善了生物活性，尽管它在细胞渗透中没有发挥重要作用。因此，这种产生三维（3D）微/纳米结构的策略可以用于体外产生功能性组织工程化构建体，所述三维（3D）微/纳米结构将增加纤维支架的孔隙率，从而改善细胞渗透。
The use of electrospun extracellular matrix (ECM)-mimicking nanofibrous scaffolds for tissue engineering is limited by poor cellular infiltration. The authors hypothesised that cell penetration could be enhanced in scaffolds by using a hierarchical structure where nano fibres are combined with micron-scale fibres while preserving the overall scaffold architecture. To assess this, we fabricated electrospun porous poly(lactic acid) (PLA) scaffolds having nanoscale, microscale and combined micro/nano architecture and evaluated the structural characteristics and biological response in detail. Although the bioactivity was intermediate to that for nanofibre and microfibre scaffold, a unique result of this study was that the micro/nano combined fibrous scaffold showed improved cell infiltration and distribution than the nanofibrous scaffold. Although the cells were found to be lining the scaffold periphery in the case of nanofibrous scaffold, micro/nano scaffolds had cells dispersed throughout the scaffold. Further, as expected, the addition of nanoparticles of hydroxyapatite (nHAp) improved the bioactivity, although it did not play a significant role in cell penetration. Thus, this strategy of creating a three-dimensional (3D) micro/nano architecture that would increase the porosity of the fibrous scaffold and thereby improving the cell penetration, can be utilised for the generation of functional tissue engineered constructs in vitro.