Increased Circulating Th17 Cells, Serum IL-17A, and IL-23 in Takayasu Arteritis.
Increased Circulating Th17 Cells, Serum IL-17A, and IL-23 in Takayasu Arteritis.
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DOI：
10.1155/2016/7841718
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发表时间：
2016
影响因子：
4
通讯作者：
Misra R
中科院分区：
文献类型：
--
作者：
Misra DP;Chaurasia S;Misra R
介绍。测量大动脉炎（TA）患者外周血中的 Th17、γδT、NK 和 NKT 细胞以及血清中的 IL-17 和 IL-23，并与疾病活动度相关。方法。采用流式细胞术对 30 名 TA 患者（ACR1990 标准）和 20 名健康对照者的外周血中 Th17（抗 CD3APC、CD4PECy7 和 IL-17PE）、NKT、NK（抗 CD3APC、CD56FITC）和 γδT（抗 CD3FITC 和 γδTCRAPC）细胞进行计数。 通过 ELISA 测定 IL-17 和 IL-23。分析与疾病活动性（NIH 标准，ITAS2010）的关系（使用非参数检验，中位数与四分位距）。结果。患者平均年龄为 33.47 ± 11.78 岁（25 名女性）；平均症状持续时间为 7.1 ± 5.3 年。 13 人未服用免疫抑制剂； 12 个处于活跃状态（ITAS2010 ≥ 4）。 TA 中 Th17 细胞的百分比显着增加（患者 2.1 (1.5–3.2) 对比对照组 0.75 (0.32–1.2)；p < 0.0001），其他细胞群没有差异。患者（分别为 6.2 (4.6–8.5) 和 15 (14.9–26.5)）的血清 IL-17 和 IL-23 (pg/mL) 显着高于对照组（分别为 3.9 (3.9–7.3) 和不可检测的中值）（p < 0.001）。亚组分析显示Th17细胞、血清IL-17和IL-23与疾病活动度或药物治疗无相关性，用药前后也无显着差异。结论。与健康对照相比，TA 患者的 Th17 细胞显着扩增，血清 IL-17 和 IL-23 水平升高。
Introduction. Th17, γδT, NK, and NKT cells in peripheral blood and serum IL-17 and IL-23 in Takayasu arteritis (TA) were measured and correlated with disease activity. Methods. Th17 (anti-CD3APC, CD4PECy7, and IL-17PE), NKT, NK (anti-CD3APC, CD56FITC), and γδT (anti-CD3FITC and γδTCRAPC) cells were enumerated by flow cytometry in peripheral blood of 30 patients with TA (ACR1990 criteria) and 20 healthy controls, serum IL-17 and IL-23 measured by ELISA. Relation with disease activity (NIH criteria, ITAS2010) was analyzed (using nonparametric tests, median with interquartile range). Results. Mean age of patients was 33.47 ± 11.78 years (25 females); mean symptom duration was 7.1 ± 5.3 years. 13 were not on immunosuppressants; 12 were active (ITAS2010 ≥ 4). The percentage of Th17 cells was significantly expanded in TA (patients 2.1 (1.5–3.2) versus controls 0.75 (0.32–1.2); p < 0.0001) with no differences in other cell populations. Serum IL-17 and IL-23 (pg/mL) in patients (6.2 (4.6–8.5) and 15 (14.9–26.5), resp.) were significantly higher (p < 0.001) than controls (3.9 (3.9–7.3) and undetectable median value, resp.). Subgroup analysis revealed no correlation of Th17 cells, serum IL-17, and IL-23 with disease activity or medications, nor any significant difference before and after medication. Conclusions. There is significant expansion of Th17 cells and elevated serum IL-17 and IL-23 levels in TA patients compared to healthy controls.