Atomic force microscopy in microbiology: new structural and functional insights into the microbial cell surface.
Atomic force microscopy in microbiology: new structural and functional insights into the microbial cell surface.
复制标题
微生物学中的原子力显微镜：对微生物细胞表面的新结构和功能见解。
DOI：
10.1128/mbio.01363-14
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发表时间：
2014-07-22
期刊：
影响因子：
6.4
通讯作者：
Dufrêne YF
中科院分区：
文献类型：
--
作者：
Dufrêne YF
微生物细胞利用其表面成分感知环境并对其做出反应。因此，了解细胞表面分子的组装和生物物理特性是一个重要的研究课题。原子力显微镜 (AFM) 能够以纳米分辨率观察活微生物细胞并操纵单细胞表面分子，已成为微生物学中的强大工具。在这里，我们调查了使用 AFM 技术在细胞表面微生物学方面取得的重大突破，强调了最新的结构和功能见解。
Microbial cells sense and respond to their environment using their surface constituents. Therefore, understanding the assembly and biophysical properties of cell surface molecules is an important research topic. With its ability to observe living microbial cells at nanometer resolution and to manipulate single-cell surface molecules, atomic force microscopy (AFM) has emerged as a powerful tool in microbiology. Here, we survey major breakthroughs made in cell surface microbiology using AFM techniques, emphasizing the most recent structural and functional insights.