Monospecific antibodies against the three mammalian fast limb myosin heavy chains
Monospecific antibodies against the three mammalian fast limb myosin heavy chains
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DOI：
10.1006/bbrc.2000.2768
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发表时间：
2000-05-27
影响因子：
3.1
通讯作者：
Hoh, JFY
中科院分区：
文献类型：
--
作者：
Lucas, CA;Kang, LHD;Hoh, JFY
哺乳动物肢体肌肉中的骨骼肌纤维有四种类型：慢肌、2A、2X和2B，每一种都具有不同的肌球蛋白重链(MyHC)亚型。现有的针对FAST MyHC的单抗缺乏跨物种的纤维类型特异性，并且不能肯定地识别2X纤维。在这项工作中，针对每个快速的MyHC提高了单抗。免疫印迹和免疫组织化学结果表明，这些单抗在大鼠体内具有单特异性。以大鼠胫骨前肌为例，说明了这些单抗用于识别三种快速纤维类型和表达多种异构体的杂交纤维的优势。免疫组织化学分析证实这些单抗在以下其他物种中具有单特异性：小鼠、豚鼠、兔子、猫和狒狒。在猫和狒狒的四肢肌肉中没有2B纤维。这些单抗构成了一套研究肌肉纤维类型及其转化的强大工具。(C)2000年学术出版社。
Skeletal muscle fibres in mammalian limb muscles are of four types: slow, 2A, 2X, and 2B, each characterized by a distinct myosin heavy chain (MyHC) isoform. Existing monoclonal antibodies (mabs) against fast MyHCs lack fibre-type specificity across species and could not positively identify 2X fibres. In this work, mabs were raised against each of the fast MyHCs. These mabs were shown to be monospecific by Western blots and immunohistochemistry in the rat. The advantages of using these mabs for identifying the three fast fibre types and hybrid fibres expressing multiple isoforms were illustrated using rat tibialis anterior muscle. Immunohistochemical analyses confirmed the monospecificity of these mabs in the following additional species: mouse, guinea pig, rabbit, cat, and baboon. 2B fibres were absent in limb muscles of the cat and baboon. These mabs constitute a set of powerful tools for studying muscle fibre types and their transformations. (C) 2000 Academic Press.