Blackberry, black raspberry, blueberry, cranberry, red raspberry, and strawberry extracts inhibit growth and stimulate apoptosis of human cancer cells in vitro
Blackberry, black raspberry, blueberry, cranberry, red raspberry, and strawberry extracts inhibit growth and stimulate apoptosis of human cancer cells in vitro
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DOI：
10.1021/jf061750g
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发表时间：
2006-12-13
影响因子：
6.1
通讯作者：
Heber, David
中科院分区：
文献类型：
--
作者：
Seeram, Navindra P.;Adams, Lynn S.;Heber, David
浆果类水果在我们的饮食中广泛食用，并因其潜在的人类健康益处而备受关注。浆果含有多种具有生物特性的植物化学物质，如抗氧化、抗癌、抗神经退行性和抗炎活性。在当前的研究中，使用高效液相色谱、紫外 (HPLC-UV) 和电喷雾电离质谱 (LC-ESI-MS) 检测评估了六种常用浆果（黑莓、黑树莓、蓝莓、蔓越莓、红树莓和草莓）的提取物中的酚类成分。浆果酚类物质的主要类别是花青素、黄酮醇、黄烷醇、鞣花单宁、棓单宁、原花青素和酚酸。评估了浆果提取物在浓度范围为 25 至 200 Ag/mL 时抑制人类口腔 (KB、CAL-27)、乳腺癌 (MCF-7)、结肠 (HT-29、HCT116) 和前列腺 (LNCaP) 肿瘤细胞系生长的能力。随着浆果提取物浓度的增加，观察到所有细胞系对细胞增殖的抑制作用增强，且细胞系之间的效力程度不同。还评估了浆果提取物刺激表达 COX-2 的结肠癌细胞系 HT-29 凋亡的能力。黑覆盆子和草莓提取物对该细胞系表现出最显着的促凋亡作用。当前研究和其他实验室提供的数据值得使用体内模型进一步研究浆果的化学预防和化疗作用。
Berry fruits are widely consumed in our diet and have attracted much attention due to their potential human health benefits. Berries contain a diverse range of phytochemicals with biological properties such as antioxidant, anticancer, anti-neurodegerative, and anti-inflammatory activities. In the current study, extracts of six popularly consumed berries-blackberry, black raspberry, blueberry, cranberry, red raspberry and strawberry-were evaluated for their phenolic constituents using high performance liquid chromatography with ultraviolet (HPLC-UV) and electrospray ionization mass spectrometry (LC-ESI-MS) detection. The major classes of berry phenolics were anthocyanins, flavonols, flavanols, ellagitannins, gallotannins, proanthocyanidins, and phenolic acids. The berry extracts were evaluated for their ability to inhibit the growth of human oral (KB, CAL-27), breast (MCF-7), colon (HT-29, HCT116), and prostate (LNCaP) tumor cell lines at concentrations ranging from 25 to 200 Ag/mL. With increasing concentration of berry extract, increasing inhibition of cell proliferation in all of the cell lines were observed, with different degrees of potency between cell lines. The berry extracts were also evaluated for their ability to stimulate apoptosis of the COX-2 expressing colon cancer cell line, HT-29. Black raspberry and strawberry extracts showed the most significant pro-apoptotic effects against this cell line. The data provided by the current study and from other laboratories warrants further investigation into the chemopreventive and chemotherapeutic effects of berries using in vivo models.