Phosphatidic acid phospholipase A1 mediates ER-Golgi transit of a family of G protein-coupled receptors.
Phosphatidic acid phospholipase A1 mediates ER-Golgi transit of a family of G protein-coupled receptors.
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DOI：
10.1083/jcb.201405020
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发表时间：
2014-07-07
期刊：
影响因子：
--
通讯作者：
Acharya JK
中科院分区：
文献类型：
--
作者：
Kunduri G;Yuan C;Parthibane V;Nyswaner KM;Kanwar R;Nagashima K;Britt SG;Mehta N;Kotu V;Porterfield M;Tiemeyer M;Dolph PJ;Acharya U;Acharya JK
胞浆磷脂酸磷脂酶A1与COPII蛋白家族成员相互作用，并且是GPCR的顺行运输所必需的。外壳蛋白II（COPII）包被的囊泡系统将新合成的分泌蛋白和膜蛋白从内质网（ER）转运到高尔基复合体。招募货物进入COPII囊泡需要COPII蛋白直接与货物分子或与货物受体进行顺行运输的相互作用。我们发现，胞浆磷脂酸磷脂酶A1（PAPLA 1）与COPII蛋白家族成员相互作用，并需要运输的Rh 1（视紫红质1），N-糖基化的G蛋白偶联受体（GPCR），从ER的高尔基复合体。在papla 1突变体中，在没有运输到高尔基体的情况下，Rh 1被异常糖基化并被错误定位。这些缺陷导致蛋白质水平降低和感光器对光的敏感性降低。几种GPCR，包括其他视紫红质和七无新娘，也受到类似的影响。我们的研究结果表明，一个胞质蛋白是必要的选择性跨膜受体货物转运的COPII外套顺行运输。
Cytosolic phosphatidic acid phospholipase A1 interacts with COPII protein family members and is required for the anterograde trafficking of GPCRs. The coat protein II (COPII)–coated vesicular system transports newly synthesized secretory and membrane proteins from the endoplasmic reticulum (ER) to the Golgi complex. Recruitment of cargo into COPII vesicles requires an interaction of COPII proteins either with the cargo molecules directly or with cargo receptors for anterograde trafficking. We show that cytosolic phosphatidic acid phospholipase A1 (PAPLA1) interacts with COPII protein family members and is required for the transport of Rh1 (rhodopsin 1), an N-glycosylated G protein–coupled receptor (GPCR), from the ER to the Golgi complex. In papla1 mutants, in the absence of transport to the Golgi, Rh1 is aberrantly glycosylated and is mislocalized. These defects lead to decreased levels of the protein and decreased sensitivity of the photoreceptors to light. Several GPCRs, including other rhodopsins and Bride of sevenless, are similarly affected. Our findings show that a cytosolic protein is necessary for transit of selective transmembrane receptor cargo by the COPII coat for anterograde trafficking.
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DOI：
10.1073/pnas.0807833105
发表时间：
2008-10-07
影响因子：
11.1
作者：
Kohyama-Koganeya, Ayako;Kim, Yeon-Jeong;Hirabayashi, Yoshio
通讯作者：
Hirabayashi, Yoshio
影响因子：
56.9
作者：
Acharya, U;Patel, S;Acharya, JK
通讯作者：
Acharya, JK
影响因子：
64.5
作者：
COLLEY, NJ;BAKER, EK;ZUKER, CS
通讯作者：
ZUKER, CS
影响因子：
64.5
作者：
BALCH, WE;MCCAFFERY, JM;FARQUHAR, MG
通讯作者：
FARQUHAR, MG
DOI：
10.1073/pnas.0535280100
发表时间：
2003-03-04
影响因子：
11.1
作者：
Gong, WJ;Golic, KG
通讯作者：
Golic, KG