Fluorescence Detection of Intron Lariat RNA with Reduction-Triggered Fluorescent Probes
Fluorescence Detection of Intron Lariat RNA with Reduction-Triggered Fluorescent Probes
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DOI：
10.1002/anie.201104425
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发表时间：
2011-01-01
影响因子：
16.6
通讯作者：
Ito, Yoshihiro
中科院分区：
文献类型：
--
作者：
Furukawa, Kazuhiro;Abe, Hiroshi;Ito, Yoshihiro
邻近荧光：带有荧光素衍生物或三苯基膦的还原触发的荧光探针在IRNA的分支位点特异性地结合序列。两种底物之间的化学反应导致未淬灭的荧光素的形成和荧光信号的发射（见图）。用这种方法可以区分前叶和叶的结构。
Fluorescence through proximity: Reduction-triggered fluorescent probes bearing either a fluorescein derivative or triphenylphosphine bind sequence-specifically at the branch site of lariat RNA. The chemical reaction between the two substrates leads to the formation of unquenched fluorescein and the emission of a fluorescence signal (see picture). Prelariat and lariat structures can be distinguished by using this method.