A comparative analysis of wet and dry ashing techniques for the extraction of phytoliths from plant material
A comparative analysis of wet and dry ashing techniques for the extraction of phytoliths from plant material
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DOI：
10.1006/jasc.2000.0623
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发表时间：
2001-08-01
影响因子：
2.8
通讯作者：
Boyd, WE
中科院分区：
文献类型：
--
作者：
Parr, JF;Lentfer, CJ;Boyd, WE
从植物材料中提取植硅体，作为考古学植硅体样品分析的参考，通常使用两种方法：（1）灰迹法或干灰化法;（2）酸浸法或湿灰化法。有人建议，这些技术可以以不同的方式修改所得的样品。特别是干灰化，被认为是在大于或等于450（0）℃下焚烧时植硅体组合收缩和翘曲的原因。干灰化和湿灰化方法之间的形态测定比较分析的结果不支持这些说法。本研究建立的差异，尺寸和曲率的短bilobate植硅体和细长的植硅体都受到干和湿灰制备的模式没有统计学意义。因此，这些方法没有可检测到的形态学影响证据。这一发现意味着，任何差异，确实发生在植硅石的大小和曲率是典型的，可能是随机排列组合内，或者说，他们是叶细胞结构的变化，而不是一个特定的解脱过程的后果的结果。这表明，使用不同方法制备化石分析参考样品的做法可以可靠地继续下去。
Two methods are commonly used for the extraction of phytoliths from plant material to be used as reference int he analysis of archaeological phytolith samples: (1) spodograms or dry ashings; and (2) acid digestions or wet ashing. It has been suggested that these techniques may modify the resultant samples in different ways. Dry ashing, in particular, has been implicated as a cause of shrinkage and warping in phytolith assemblages when incineration occurs at greater than or equal to 450(0)C. The results of a morphometric comparative analysis between the dry ashing and wet ashing methods do not support these claims. This study establishes the differences in patterns of dimension and curvature of short bilobate phytoliths and of elongate phytoliths both subjected to dry and wet ash preparation are not statistically significant. There is, therefore, no detectable evidence of morphological impact as a result of these methods. This finding implies that any difference that do occur in phytolith size and curvature are typical, possibly random permutation within assemblages, or that they are the result of variation in leaf cell structure rather than the consequence of a particular extrication procedure. This suggests that the practice of using different methods of preparation of reference samples for fossil analysis can be reliably continued.