Ad4BP regulating steroidogenic P-450 gene is a member of steroid hormone receptor superfamily.
Ad4BP regulating steroidogenic P-450 gene is a member of steroid hormone receptor superfamily.
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Ad4BP 调节类固醇生成 P-450 基因是类固醇激素受体超家族的成员。
DOI：
10.1016/s0021-9258(18)53202-6
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
T. Omura
中科院分区：
文献类型：
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作者：
S. Honda;K. Morohashi;M. Nomura;H. Takeya;M. Kitajima;T. Omura
牛细胞色素P-450(11β)基因(CYP11B)在启动子区域有Ad1、Ad2、Ad3、Ad4、Ad5和Ad6六个不同的顺式作用元件。Ad4位点也存在于其他类固醇合成基因P-450的启动子区域以及细胞色素P11B1中。从牛肾上腺皮质核提取液中分离纯化了一种与Ad4结合位点特异结合的Ad4结合蛋白(Ad4BP)，其分子质量为53 kDa。从牛肾上腺皮质基因文库中分离到编码Ad4BP的互补DNA。该克隆含有一个1383个碱基对的开放阅读框，编码461个氨基酸，计算其相对分子质量为51,020。预测的Ad4BP氨基酸序列表明，该蛋白含有一个锌指结构域和一个配体结合/二聚结构域。Ad4BP是类固醇激素受体超家族的新成员。对激素受体超家族一级结构的比较表明，Ad4BP与调控福氏Tarazu基因的FTZ-F1和在小鼠胚胎癌细胞中表达的ELP高度同源。将Ad4BP表达载体导入CV-1细胞，可激活启动子区域携带Ad4序列的CAT报告基因的转录。
Bovine cytochrome P-450(11 beta) gene (CYP11B) has six different cis-acting elements, Ad1, Ad2, Ad3, Ad4, Ad5, and Ad6, in the promoter region. The Ad4 site also exists in the promoter regions of other steroidogenic P-450 genes as well as in CYP11B1. An Ad4-binding protein (Ad4BP) which specifically binds to the Ad4 site was purified from bovine adrenal cortex nuclear extract, and its molecular mass was 53 kDa. A complementary DNA encoding Ad4BP was isolated from a bovine adrenal cortex cDNA library. The cDNA clone contained an open reading frame of 1383 base pairs encoding 461 amino acids, whose calculated molecular weight was 51,020. The predicted amino acid sequence of Ad4BP revealed that the protein has a zinc finger domain and a ligand binding/dimerization domain. Ad4BP is a novel member of the steroid hormone receptor superfamily. Comparison of the primary structures of the hormone receptor superfamily showed that Ad4BP was highly homologous to FTZ-F1, which regulates the fushi tarazu gene, and ELP, which is expressed in the murine embryonal carcinoma cells. Transfection of a Ad4BP expression plasmid into CV-1 cells activated the transcription of the CAT reporter gene carrying the Ad4 sequence in the promoter region.