Evaluation of the new body fluid mode on the Sysmex XE-5000 for counting leukocytes and erythrocytes in cerebrospinal fluid and other body fluids
Evaluation of the new body fluid mode on the Sysmex XE-5000 for counting leukocytes and erythrocytes in cerebrospinal fluid and other body fluids
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DOI：
10.1515/cclm.2010.108
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发表时间：
2010-05-01
影响因子：
6.8
通讯作者：
Lindemans, Jan
中科院分区：
文献类型：
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作者：
de Jonge, Robert;Brouwer, Rob;Lindemans, Jan
背景资料：方法：在Sysmex XE-5000全自动生化分析仪上进行红细胞（RBC）和白色细胞（WBC）的体液（BF）模式检测。BF（差异）计数（139例患者样本和87例正常样本）进行测量，并与Fuchs-Rosenthal室和染色的细胞离心涂片进行比较。注意到使用BF模式进行的扩展细胞计数的WBC检测限（CV 20%）提高至10 × 10（6）/L。对于大多数BF，观察到与手动方法的高度一致性[脑脊液（CSF）的平均偏倚为+2至6x 10（6）/L，其他液体的平均偏倚为-1至12 x10（6）/L]。在CSF中，与手动方法相比，BF模式计数更多的WBC（多态性核细胞）（平均偏倚+5至6x 10（6）/L），尤其是在细胞计数较低的样本中（
Background: We evaluated the body fluid (BF) mode on the new Sysmex XE-5000 analyzer.Methods: Red (RBC) and white blood cell (WBC) (differential) counts of BFs (139 patient samples and 87 normal samples) were measured and compared to the Fuchs-Rosenthal chamber and stained cytospin slides.Results: Extended cell counting using the BF mode was noted to have an improved WBC detection limit (CV20%) of 10x10(6)/L. Excellent agreement with the manual method was observed for most BFs [mean bias +2 to 6x10(6)/L for cerebrospinal fluid (CSF) and -1 to 12x10(6)/L for other fluids]. In CSF, the BF-mode counted more WBC (polymorphic nuclear cells) compared with the manual method (mean bias +5 to 6x10(6)/L), especially in samples with low cell counts (